【摘要】 目的观察糖脂平对喂养型胰岛素抵抗大鼠血清肿瘤坏死因子-α(TNF-α)水平及脂肪组织mRNA表达的影响,探讨其抗动脉粥样硬化(AS)的作用机制。方法将28只清洁级SD大鼠随机分为对照组、模型组、中药组和西药组,采用高脂高盐饲料喂养,造模成功后分别给予生理盐水、糖脂平和罗格列酮灌胃。给药8周后,用放免法测定各组大鼠血清TNF-α水平,反转录-聚合酶链反应(RT-PCR)方法测定附睾旁脂肪组织TNF-αmRNA表达水平。结果模型组大鼠血清TNF-α水平为(1.78±0.13)ng/mL,较对照组大鼠明显升高,糖脂平或罗格列酮灌胃可分别使模型大鼠血清TNF-α水平降至(1.46±0.13)ng/mL和(1.32±0.16)ng/mL,差异有统计学意义(P<0.01)。模型组大鼠脂肪组织TNF-αmRNA表达量较对照组显著升高(P<0.01),与模型组相比,糖脂平或罗格列酮灌胃可分别使TNF-αmRNA下降30.2%和31.6%,差异有统计学意义(P<0.01)。结论糖脂平改善胰岛素抵抗和抗AS作用可能与其降低血清TNF-α水平,抑制TNF-αmRNA过度表达有关。更多还原

【Abstract】 Objective To investigate the effect of Tangzhiping on serum TNF-α level and the mRNA expression of TNF-α in epididymal fat pads in rats with insulin resistance(IR).Methods Twenty-eight SD rats were randomly divided into control group,model group,Chinese medicine group and western medicine group.The rat model of IR was established by feeding high-fat and high-salt diet.Rats in Chinese medicine group were treated with Tangzhiping and rats in western medicine group were treated with rosiglitazone for 8 weeks.Eight weeks later,radioimmune assay was used to detect the serum level of TNF-α and RT-PCR was used to detect the mRNA expression of TNF-α in epididymal fat pads.Results Serum TNF-α level of model group was(1.78±0.13)ng/mL,which was significantly higher than that of control group.Treatment with Tangzhiping or rosiglitazone reduced serum TNF-α level to(1.46±0.13)ng/mL and(1.32±0.16)ng/mL(P<0.01),respectively.Compared with control group,TNF-α mRNA expression in rat adipose tissue was significantly higher in model group(P<0.01).After treated with Tangzhiping or rosiglitazone,TNF-α mRNA expression in adipose tissue was decreased by 30.2% and 31.6%(P<0.01),respectively.Conclusion Decreased serum level of TNF-α and downregulated TNF-α mRNA expression by Tangzhiping may contribute to its role in improving IR and protecting against atherosclerosis.更多还原