【摘要】 目的针对间接酶联免疫吸附试验(ELISA)检测日本血吸虫感染结果标准化中的几个常见问题,开发一款光密度值标准化软件,规范标准化过程和结果。方法以改良光密度值标准化变换方法(I-STOD)为开发依据,以Matlab GUI为开发工具,综合I-STOD实施过程中的各个环节以及相应的处理方法进行软件开发,并用来自日本血吸虫病疫区的血清检测结果对该软件进行测试。结果成功开发了用于标准化光密度值的软件——I-STOD V1.0。该软件的运行环境为WINDOWS XP/WIN 7,所需硬盘容量约0.5 GB。用来自日本血吸虫病疫区的血清检测结果对该软件测试表明,该软件有针对性地解决了标准化过程中的标准曲线可靠性判断、样本适用范围及超范围样本稀释度确定等问题,使得I-STOD在应用上更简便、在操作上更规范。结论基于I-STOD方法开发的I-STOD V1.0应用软件操作简便,且能有效地对间接ELISA法的实验结果进行标准化。更多还原

【Abstract】 Objective To develop a software that can be used to standardize optical density to normalize the procedures and results of standardization in order to effectively solve several problems generated during standardization of in-direct ELISA results.Methods The software was designed based on the I-STOD method with operation settings to solve the problems that one might encounter during the standardization.Matlab GUI was used as a tool for the development.The software was tested with the results of the detection of sera of persons from schistosomiasis japonica endemic areas.Results I-STOD V1.0(WINDOWS XP/WIN 7,0.5 GB) was successfully developed to standardize optical density.A serial of serum samples from schistosomiasis japonica endemic areas were used to examine the operational effects of I-STOD V1.0 software.The results indicated that the software successfully overcame several problems including reliability of standard curve,applicable scope of samples and determination of dilution for samples outside the scope,so that I-STOD was performed more conveniently and the results of standardization were more consistent.Conclusion I-STOD V1.0 is a professional software based on I-STOD.It can be easily operated and can effectively standardize the testing results of in-direct ELISA.更多还原