【摘要】 目的建立减少DNA低体积扩增过程中产生气泡的方法。方法采用激光显微切割技术、PALM系统收集目标细胞,并在1μL～1.5μL低体积扩增样本,加入扩增液0.7μL～0.8μL。结果低体积扩增反应中的失败位点比例为2.3%,比常规反应位点时常会产生气泡导致该位点样本扩增失败且达30%以上的比例显著降低。结论低体积扩增方法可以减少或克服扩增过程中气泡的产生,提高扩增成功率。更多还原

【Abstract】 Objective To establish a new method for reducing the bubbles in process of Low Volume PCR(LV-PCR).Methods Target cells were collected under Laser Capture Microscope and LV-PCR was performed.Results Bubbles can be reduced by skillful collection operations and LV-PCR amplification.Conclusion The LV-PCR method was better than standard PCR method for analysis of varying concentration DNA samples.更多还原