【摘要】 为进一步开展番鸭的遗传标记筛选提供参考。采用PCR-SSCP和直接测序技术对2个番鸭群体(天柱番鸭和白羽番鸭)脂联素基因进行了多态性检测。结果表明,引物A1检测到G167A沉默突变,位于外显子1,产生3种基因型:AA、AB和BB,但天柱番鸭群体仅检测到AA基因型;在引物A2检测到内含子T290C突变,产生3种基因型:CC、CT和TT;在引物A4检测到G711A沉默突变,位于外显子2,产生3种基因型:EE、EF和FF;在引物A3没有检测到多态。在2个番鸭群体中,A、C和E均为优势等位基因;3个SNP位点在2个番鸭群体中均处于Hardy-Weinberg平衡状态(P>0.05)。更多还原

【Abstract】 To provide references for further screening of genetic marker of Muscovy duck,the polymorphism of adiponectin gene whole coding region and intron of Tianzhu Muscovy duck and White Muscovy duck populations were detected by PCR-SSCP method and Direct sequencing technology.The results showed that G167A silent mutation of exon 1 was found in primer A1,which produced genotypes: AA,AB and BB,but AA genotype in all individuals of Tianzhu Muscovy duck.T290C mutation of intron was found in primer A2,which resulted in 3 genotypes: CC,CT and TT.G711A silent mutation was found in exon 2 and resulted in 3 genotypes(EE,EF and FF).Primer A3 had not exhibited polymorphism in different individuals.Allele A,C and E were prodominant in two Muscovy duck populations.Three SNPs frequencies were in accordance with Hardy-Weinberg equilibrium(P>0.05).更多还原