【摘要】 本研究旨在探索红细胞生成素受体基因(EPOR)影响红细胞相关性状的分子机理。对EPOR基因第1内含子和第4内含子突变位点g.705G>T和g.2373C>T,以飞行时间质谱进行个体基因型分型,在构建的大白猪×民猪F2代资源群体内开展其与6种血常规性状(红细胞压积、血红蛋白、平均红细胞血红蛋白量、平均红细胞体积、红细胞计数及红细胞分布宽度)的关联分析。结果表明,第1内含子突变位点g.705G>T未发现与血常规性状显著关联;第4内含子突变位点g.2373C>T群体内只发现2种基因型,其中CT基因型个体的红细胞压积显著高于CC基因型个体(P<0.05),但并未发现与其他性状显著关联。结果显示,EPOR基因突变可显著影响红细胞压积,说明该基因可作为影响红细胞压积的主效候选基因开展深入研究。更多还原

【Abstract】 The aim of present study was to investigate the effect of porcine erythropoietin receptor(EPOR) gene on blood parameters.Six blood parameters,hematocrit(HCT),hemoglobin(HGB),mean corpuscular hemoglobin(MCH),mean cell volume(MCV),red blood cell count(RBC) and red blood cell distribution width(RDW),were measured in Large White × Minzhu intercross porcine population.EPOR was genotyped using matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry(MALDI-TOF).The associations of g.705G>T in intron 1 and g.2373C>T in intron 4 with the six blood parameters were analyzed.The result showed that the SNP at g.705G>T was not significantly associated with any trait.Two genotypes(CC and CT) of SNP at g.2373C>T were found in this population.The HCT of individuals with CT genotype was higher than that of individuals with CC genotype(P<0.05),while this SNP was not significant associated with other five traits.These results indicate that the mutation of EPOR gene can affect the HCT and this gene can be a potential candidate gene for immunity relate traits(e.g.HCT) for further study.更多还原