【摘要】 旨在探讨日粮中添加壳聚糖对早期断奶仔猪肠道屏障功能的影响。将30头21d断奶仔猪((6.62±0.59)kg)随机分为3组,预试期采用大肠杆菌攻毒(109细胞.头-1),对照组饲喂基础日粮,试验组分别在基础日粮中添加50mg.kg-1金霉素和300mg.kg-1壳聚糖,饲养期21d。结果表明,与对照组相比,壳聚糖能降低血浆中D-乳酸(D-Lactate)含量(P<0.05)、二胺氧化酶(DAO)活性(P<0.05)和血清内皮素-1(ET-1)水平(P<0.05),但对血清一氧化氮(NO)含量无明显影响(P>0.05);免疫组化结果显示,壳聚糖组空肠黏膜Occludin(P<0.01)和ZO-1(P<0.05)蛋白表达提高;荧光定量PCR结果表明,壳聚糖组空肠黏膜Occludin(P<0.01)和ZO-1(P<0.05)mRNA表达丰度升高。结果说明,日粮中添加300mg.kg-1的壳聚糖能降低早期断奶仔猪小肠黏膜屏障功能的损伤作用。更多还原

【Abstract】 In order to explore the effects of chitosan on intestinal barrier of weanling pigs,thirty piglets with an average body weight of(6.62±0.59) kg were challenged with enterotoxigenic Escherichia coli(109 cell per head) during preliminary trial period and randomly assigned to three treatment groups,a basal diet was added in control group,50 mg·kg-1 chlortetracycline,300 mg·kg-1 chitosan were added in the experimental groups,respectively,for 21 d.The results showed that,compared with the control group,chitosan could decrease the level of D-Lactate(P<0.05) and the activity of DAO(P<0.05) in plasma and the level of ET-1(P<0.05) in serum,but it had no significant effect on the contents of NO(P>0.05) in serum.The result of immunohistochemistry showed that the protein expression levels of Occludin(P<0.01) and ZO-1(P<0.05) in piglets jejunum mucous added with chitosan were increased.The result of fluorescence quantitative PCR showed that the expression abundances of Occludin(P<0.01) and ZO-1(P<0.05) mRNA in piglets jejunum mucous added with chitosan were improved.These result indicate that diet supplemented with 300 mg·kg-1 chitosan can protect greatly the intestinal barrier of early weanling piglets from injuring.更多还原