【摘要】 目的探讨蛇床子素诱导家兔阴茎勃起的作用及其机制。方法检测蛇床子素对磷酸二酯酶5(PDE5)的抑制活性;检测蛇床子素对离体阴茎海绵体组织产生cGMP的诱导活性;建立急性脊髓损伤家兔模型,蛇床子素静脉注射给药(3mg/kg或10mg/kg),通过测量暴露的阴茎粘膜长度评价蛇床子素体内诱导勃起的功能。结果蛇床子素对PDE5直接抑制活性IC50为88.9nmol/L;对离体阴茎海绵体cGMP诱导活性ED50为58.2μmol/L;在一氧化氮供体硝普钠存在条件下,静脉注射蛇床子素对ASCI家兔模型具有剂量依赖性促进阴茎勃起作用。结论蛇床子素对勃起功能障碍具有治疗价值。更多还原

【Abstract】 Objective The aim of this study was to investigate the effects of Osthole(OST) on inducing penile erection in rabbits with an acute spinal cord injury(ASCI).Methods OST was given intravenously(3 or 10mg/kg) to conscious male albino rabbits with a surgical transection of the spinal cord at the L2-L4 lumbar vertebra.Erection was evaluated in a time-course manner by measuring the length of the uncovered penile mucosa.Results The efficacy of OST was potentiated by an intravenous injection of sodium nitroprusside,a nitric oxide donor.Potentiation of the effect by nitric oxide donor implies that OST can enhance the erectile activity during sexual arousal.Conclusion These results suggest that OST may be useful for treating erectile dysfunction.更多还原