【摘要】 目的对hsa-miR-335进行系统的生物信息学分析,预测hsa-miR-335可能参与的生物学过程,为本研究小组深入研究其在脂肪细胞分化中的功能和机制奠定基础。方法通过miRbase获取并分析hsa-miR-335序列特征;应用TargetScan,PicTar及miRanda预测hsa-miR-335的靶基因,并结合已证实的靶标基因,进行功能注释(Gene Ontol-ogy)和Pathway富集分析(Pathway Enrichment)。应用NCBI Mapviewer、UCSC Genome Browser等工具对hsa-miR-335进行启动子相关预测。结果 miR-335在各物种之间具有高度保守性,其靶基因功能富集于胰腺外分泌、脂质激酶活性调控、wnt受体信号的调控、细胞周期等生物学过程(P>0.01),其靶基因信号通路富集于促性腺激素释放激素信号通路、MAPK信号通路、胰岛素信号通路、细胞周期等信号转导通路(P<0.05)。启动子预测提示hsa-miR-335为基因内miRNA,在基因组上下游10kb以内存在CpG岛,转录起始位置(TSS)、Poly A信号、转录因子结合位置(TFBS)。结论 hsa-miR-335预测的靶基因集合富集于多个生物学过程,与肥胖密切相关,并可能存在其独立的转录调控机制。为后续miR-335在肥胖人脂肪细胞中生物学功能研究奠定基础。更多还原

【Abstract】 Objective To predict the biological process that hsa-miR-335 might be involved in by a series of bioinformatics analysis,so as to lay foundations and provide theoretical basis for the further studies of hsa-miR-335 biological function in human preadipocyte.Methods The sequence of hsa-miR-335 was got from miRBase database,and target genes of hsa-miR-335 was predicted by TargetScan,PicTar and miRanda,and then analyzed by GO(gene ontology) and pathway Enrichment.Meanwhile,we predicted the possible promoter of miR-335 via transcription start sites(TSS),CpG Island,ployA signal,EST,transcription factor binding sites(TFBS) and conserved sequence.Results The functions of the target genes were enriched in exocrine pancreas development,regulation of lipid kinase activity,Wnt receptor signaling pathway through beta-catenin,cell cycle,and other biological processes(P>0.01).The GnRH signaling,MAPK signaling,insulin signaling,and cell cycle signal transduction pathways were significantly enriched(P<0.05).MiR-335 was introgenic miRNA.TSS,CpG Island,ployA signal,TFBS existed in the genome upstream and downstream within 10kb.Conclusion The target genes set of hsa-miR-335 enrich in multiple biological process were related with the obesity,hsa-miR-335 located in the intron of MEST may have its own transcription unit,which laying foundations for the further study in human preadipocytes.更多还原