【摘要】 [目的]探讨蛋白酶体抑制剂硼替佐米(bortezomib)对K562细胞株DNA甲基转移酶(DNMTs)表达、细胞凋亡的影响。[方法]以不同浓度的硼替佐米(0、6、20、60nmol/L)处理白血病K562细胞株,Western blot法检测细胞内DNMT1的表达,流式细胞术检测细胞凋亡。[结果]与对照组相比,硼替佐米可显著抑制DNMT1表达;硼替佐米作用于细胞12、24、36h后细胞凋亡率逐渐增加,60nmol/L硼替佐米作用36h后细胞凋亡率最高,为61.68%±3.20%。[结论]硼替佐米抑制K562细胞株DNMT1表达,并诱导细胞凋亡,呈浓度依赖性。更多还原

【Abstract】 [Purpose] To investigate the effects of bortezomib on the expression of DNA methyltransferase(DNMTs) and on cell apoptosis in K562 cell line.[Methods] K562 cells were treated by bortezomib with the concentrations of 0,6,20 and 60nmol/L respectively.Western blot was used to analyze the intracellular expression of DNMT1,and flow cytometry was used to detect cell apoptosis.[Results] Compared with control group,the expression of DNMT1 decreased when treated with Bortezomib.Along with Bortezomib for 12,24,36h,the apoptosis increased gradually,and reached the highest after treating with 60nmol/L Bortezomib for 36h.[Conclusion] Bortezomib can suppress the expression of DNMT1 and induce apoptosis of K562 cells with concentration-dependence.更多还原