【摘要】 背景:研究报道骨髓间充质干细胞移植可用于治疗急性肾损害,人羊膜间充质干细胞在其他组织器官损伤修复中具有良好的治疗作用。目的:观察人羊膜间充质干细胞移植对顺铂诱导小鼠急性肾损害的治疗作用。方法:分离、培养、鉴定人羊膜间充质干细胞。雌性ICR小鼠随机被分为3组,除空白组外,其余均诱导小鼠急性肾损害模型。人羊膜间充质干细胞移植组第8天尾静脉缓慢注射1.0×1010L-1浓度的人羊膜间充质干细胞0.3mL;模型组和空白组尾静脉均注入等量生理盐水。结果与结论:顺铂诱导急性肾损害小鼠人羊膜间充质干细胞移植17d,生化指标检测结果显示,与模型组比较,人羊膜间充质干细胞移植组小鼠血清肌酐和血尿素氮水平明显降低,且已达空白组水平。肾组织苏木精-伊红染色后,组织病理检查可见人羊膜间充质干细胞移植组小鼠,肾组织结构清晰、完整,肾小管丰富,完整性和结构与空白组相似,较模型组病理学变化改善明显。人羊膜间充质干细胞移植7d,双色荧光染色可见大量人羊膜间充质干细胞在肾组织中有大量定植和分布,肾小管上皮细胞中也有少量人羊膜间充质干细胞来源的MAB1281-FITC阳性细胞分布。结果表明,人羊膜间充质干细胞移植可促进急性肾损害的修复。更多还原

【Abstract】 BACKGROUND: Recent researches have reported that bone marrow mesenchymal stem cells transplantation can be used for the treatment of acute kidney injury, and human amnion-derived mesenchymal stem cells have a good therapeutic effect in repairing other tissues and organs injury. OBJECTIVE: To investigate the effect of the human amnion-derived mesenchymal stem cells transplantation on cisplatin-induced acute kidney injury. METHODS: Human amnion-derived mesenchymal stem cells were isolated, cultured and identified in vitro. Male ICR mice were randomly divided into three groups: normal control group, model group, human amnion-derived mesenchymal stem cells group, and mices in the model group and human amnion-derived mesenchymal stem cells group were used to establish acute kidney injury models. In the human amnion-derived mesenchymal stem cells group, 0.3 mL human amnion-derived mesenchymal stem cells with the concentration of 1.0×1010/L were injected into the mice via tail vein at 8 days; mice in the model group and normal control group were injected with normal saline in the same dose. RESULTS AND CONCLUSION: At 17 days after the establishment of cisplatin-induced acute kidney injury model by human amnion-derived mesenchymal stem cells transplantation, renal function examination results demonstrated that compared with model group, the blood urea nitrogen and serum creatinine levels in human amnion-derived mesenchymal stem cells group were significantly increased, and reached to the levels in normal control group. Hematoxylin-eosin staining results showed the clear and complete renal organizational structure with rich tubules in the human amnion-derived mesenchymal stem cells group, the integrity and structure were similar to those of the mice in the normal control group, but the pathological changes of the human amnion-derived mesenchymal stem cells group were significantly improved when compared with model group. At 7 days after human amnion-derived mesenchymal stem cells transplantation, double labeling immunofluorescence results showed there was a large number of human amnion-derived mesenchymal stem cells colonization and distributionin in the nephridial tissue, a few MAB1281-FITC positive cells derived from human amnion-derived mesenchymal stem cells also found in the epithelium of kidney tubules. The human amnion-derived mesenchymal stem cells transplantation could promote the recovery of acute kidney injury.更多还原