【摘要】 背景:咖啡酸对破骨细胞形成分化方面影响的研究很少,对破骨细胞性骨吸收机制的研究更少。目的:观察咖啡酸对破骨细胞形成分化及组织蛋白酶K基因表达的影响,分析甘薯提取物咖啡酸对破骨细胞性骨吸收的抑制机制。方法:采用3种不同细胞培养系,观察咖啡酸对破骨细胞形成分化的影响,同时利用反转录-聚合酶链反应技术观察咖啡酸对组织蛋白酶K基因表达的抑制作用。结果与结论:咖啡酸在3种不同细胞培养系中均显示出直接抑制破骨细胞形成和分化的作用,根据半定量反转录-聚合酶链反应基因表达分析,咖啡酸对RANKL受体RANKmRNA的表达完全无影响,但显著抑制了组织蛋白酶K的表达。提示甘薯提取物有效成分咖啡酸不仅能直接抑制破骨细胞的形成及分化,而且可显著抑制组织蛋白酶K的表达,其可能是甘薯提取物抑制破骨细胞性骨吸收的作用机制。更多还原

【Abstract】 BACKGROUND:To date,there are few researches on the effect of caffeic acid on the formation and differentiation of osteoclasts,and fewer researches on the mechanism of osteoclastic bone resorption.OBJECTIVE:To explore the mechanism of sweet potato extract caffeic acid inhibiting osteoclastic bone resorption by studying the effect of caffeic acid on the formation and differentiation of osteoclast and on the expression of cathepsin K gene.METHODS:Caffeic acid effects on formation and differentiation of osteoclasts in three osteoclast cell lines were observed and the inhibition effect of caffeic acid on the expression of cathepsin K gene was detected by reverse transcription-PCR.RESULTS AND CONCLUSION:Caffeic acid could directly inhibit the formation and differentiation of osteoclasts in three osteoclast cell lines.Caffeic acid had no influences on the expression of receptor activator of nuclear factor-kappa B ligand and receptor activator of nuclear factor-kappa B mRNA,but could markedly inhibit the expression of cathepsin K gene based on the semi-quantitative gene analysis of reverse transcription-PCR.Caffeic acid,an effective component of sweet potato extract,cannot only directly inhibit the formation and differentiation of osteoclast,but also markedly inhibit the expression of cathepsin K gene,which seems to be the mechanism of sweet potato extract inhibiting osteoclastic bone resorption.更多还原