【摘要】 背景:有研究表明重组人表皮生长因子能促进烧伤创面愈合,内源性表皮生长因子通过与表皮生长因子受体结合发挥生物学效应。目的:观察局部应用重组人表皮生长因子对大鼠烫伤创面表皮生长因子受体的影响,分析其促进创面愈合的可能机制。方法:制备Wistar大鼠背部深Ⅱ度烫伤模型,分为2组,对照组于创面喷洒生理盐水,实验组喷洒重组人表皮生长因子。烫伤后0,1,3,5,7,10,14,21d取创面组织,采用Western blot检测表皮生长因子受体蛋白表达,并测定两组大鼠创面愈合率。结果与结论:烫伤后7-21d实验组创面愈合率高于对照组(P<0.05)。两组表皮生长因子受体蛋白表达在1d明显降低(P<0.05),随后又升高,7d达峰值且对照组比实验组高(P<0.05);峰值过后,对照组逐渐下降,21d接近烫伤前水平,实验组迅速下降,14d达谷值,随后上升,21d接近烫伤前水平,两组差异有显著性意义(P<0.05)。说明早期局部应用重组人表皮生长因子可影响表皮生长因子受体的表达规律,并显著促进大鼠深Ⅱ度烫伤创面愈合。更多还原

【Abstract】 BACKGROUND:Previous studies have demonstrated that recombinant human epidermal growth factors can obviously accelerate wound closure on burning wound,and endogenous epidermal growth factor induces biological effects in combination with epidermal growth factor receptor.OBJECTIVE:To investigate the effect of local recombinant human epidermal growth factors on the expression of epidermal growth factor receptor in the healing of scald wound in rats.METHODS:Wistar rats were prepared for back deep Ⅱdegree scald models and divided into two groups.Rats in the control group were sprayed physiological saline and those in the experimental group were sprayed recombinant human epidermal growth factors.The healing rate of the wound was observed and expression of epidermal growth factor receptor protein was determined by Western-blot at 0,1,3,5,7,10,14,21 days after operation.RESULTS AND CONCLUSION:The wound healing rates of the experimental group were higher than those of the control group from 7 to 21 days(P<0.05).The expression of epidermal growth factor receptor in the two groups were reduced at 1 day,and raised to the peak level at 7 days,which was higher in the control group than the experimental group(P<0.05).After the peak level,the expression of epidermal growth factor receptor in the control group was reduced slowly to the normal level.While,the expression of epidermal growth factor receptor in the experimental group was reduced rapidly to a minimum at 14 days;after that,it was raised slowly and closed to the normal level.The two groups showed significant differences(P<0.05).These findings indicate that local application of recombinant human epidermal growth factors at the early phase of wound healing can produce an influence on the expression of epidermal growth factor receptor and obviously accelerate wound closure in deep Ⅱscald wound rats.更多还原