【摘要】 背景:研究表明镁参与了机体免疫系统的调节,包括细胞增殖、凋亡等过程。目前国内外尚未见报道镁浓度对健康人外周血CD4+T淋巴细胞的凋亡和自噬有何影响。目的:拟观察镁离子浓度对体外培养的健康人外周血CD4+T淋巴细胞凋亡和自噬的影响。方法:分离20名健康人外周血CD4+T淋巴细胞,与不同浓度的镁共培养24-72h,分别为低浓度镁(0.2和0.4mmol/L)、生理浓度镁(0.8mmol/L)和高浓度镁(5和10mmol/L),然后以流式细胞术检测细胞的凋亡和自噬水平。结果与结论:①CD4+T细胞与低浓度镁共培养后,细胞的凋亡和自噬水平均显著增加(P<0.05)。②高浓度镁则显著下调CD4+T细胞的凋亡和自噬水平(P<0.05)。③非生理浓度的镁对CD4+T细胞凋亡和自噬的影响呈浓度依赖性和时间依赖性,但是生理浓度镁(0.8mmol/L)对健康人外周血CD4+T细胞的凋亡和自噬无影响(P>0.05)。结果提示细胞外镁缺乏可以诱导健康人外周血CD4+T细胞的凋亡和自噬,而补充镁可以下调其凋亡和自噬水平,生理浓度的镁不影响其凋亡和自噬水平。更多还原

【Abstract】 BACKGROUND:Studies have demonstrated that magnesium(Mg) participates in the regulation of immune system including cell proliferation and apoptosis.Few studies have been reported regarding the effects of Mg concentration on the apoptosis and autography of CD4+ T lymphocytes in peripheral blood from healthy subjects.OBJECTIVE:To investigate the effects of Mg concentration on the apoptosis and autography of CD4+ T lymphocytes in peripheral blood from healthy subjects.METHODS:Peripheral blood CD4+T lymphocytes were isolated from 20 healthy subjects,and then cultured in low concentrations of Mg(0.2 and 0.4 mmol/L),physiological concentration of Mg(0.8 mmol/L) or high concentration of Mg(5 and 10 mmol/L).Apoptosis and autophagy levels were analyzed by flow cytometry after 1,2,and 3 days.RESULTS AND CONCLUSION:The apoptosis and autophagy level of CD4+ T cells were significantly increased after cultured with low concentrations of extracellular Mg(0.2 and 0.4 mmol/L)(P < 0.05) and significantly decreased after cultured with high concentrations of extracellular Mg(5 and 10 mmol/L)(P < 0.05).Mg at a non-physiological concentration showed a concentration-dependent and time-dependent effect on apoptosis and autophagy of CD4+ T cells,but Mg at a physiological concentration(0.8 mmol/L) exhibited no effects on the apoptosis and autography of CD4+ T cells in the peripheral blood from healthy subjects(P > 0.05).Extracellular Mg deficiency exacerbated the ratio of apoptosis and autophgy in cultured peripheral CD4+ T cells from healthy subjects.Mg supplement reduced them and physiological concentration of Mg did not affect them.更多还原