【摘要】 背景:制备脐血血清体外扩增、培养间充质干细胞成为目前的研究热点,但尚无不同体积分数脐血血清对骨髓间充质干细胞的增殖能力的影响的报道。目的:观察比较不同体积分数的脐血血清对骨髓间充质干细胞的增殖能力的影响。方法:无菌条件下取20例人骨髓标本,采用密度梯度离心法提取单个核细胞,分别用体积分数为5%,7.5%,10%,15%,20%的脐血血清+DMEM/F12培养基,行骨髓间充质细胞培养,取第3代细胞,用MTT法观察不同体积分数的脐血血清对间充质干细胞的增殖能力的影响。结果与结论:在体积分数5%,7.5%,10%的脐血血清+DMEM/F12培养基组,细胞增殖能力较佳,明显优于体积分数15%,20%的脐血血清+DMEM/F12培养基组,差异有显著性意义(P<0.05)。说明在较低体积分数脐血血清培养体系中,骨髓间充质干细胞增殖状态活跃。更多还原

【Abstract】 BACKGROUND:Culture of bone marrow mesenchymal stem cells(BMSCs) after preparation of umbilical cord serum has become an increasing research area.However,there have been no reports regarding the effects of different volume fractions of umbilical cord serum on the proliferation ability of BMSCs.OBJECTIVE:To compare the effects of different volume fractions of umbilical cord serum on the proliferation ability of BMSCs.METHODS:Twenty bone marrow samples were obtained under aseptic conditions.The mononuclear cells were extracted by density gradient centrifugation method,and cultured with 5%,7.5%,10%,15%,20% umbilical cord serum + DMEM/F12 culture medium respectively.Passage 3 BMSCs were included in this study.MTT assay was used to investigate the effects of different volume fractions of umbilical cord serum on the proliferation ability of BMSCs.RESULTS AND CONCLUSION:Compared with the 15%,20% volume fractions of umbilical cord serum + DMEM/F12 medium groups,the proliferation ability of BMSCs was significantly stronger than in the 5%,7.5%,10% volume fractions of umbilical cord serum + DMEM/F12 medium groups(P < 0.05).These findings suggest that the proliferation ability of BMSCs is more active at low volume fractions of human umbilical cord serum culture system.更多还原