【摘要】 背景:周围神经缺损修复是临床上一大难题,由于自体神经移植有一定的局限性,人工神经修复材料是一种很有前途的选择。目的:探讨静电纺丝聚乳酸聚乙醇酸共聚物(PLGA)-丝素-胶原纳米神经导管修复大鼠坐骨神经缺损的可能性。方法:雌性SD大鼠36只,制备约10mm的坐骨神经缺损,分别采用倒转自体神经、静电纺丝PLGA-丝素-胶原神经导管、单纯PLGA神经导管桥接,术后12周进行大体观察、神经电生理测定、光镜观察、透射电镜观察和图像分析对比,了解神经再生的情况。结果与结论:静电纺丝法制备成的纳米神经导管管壁疏松多孔,能够模拟细胞外基质的结构。静电纺丝PLGA-丝素-胶原神经导管组在促进坐骨神经再生、提高再生神经髓鞘化、加速再生神经功能重建等方面均优于单纯PLGA导管组,比自体神经移植组略差。更多还原

【Abstract】 BACKGROUND:The repair of peripheral nerve defects exhibits a clinical challenge.Because of the limitation of nerve autograft,it is a promising choice for artificial nerve grafts.OBJECTIVE:To investigate the possibility of repairing sciatic nerve defects by electrostatic spinning poly (lactic-co-glycolic acid) (PLGA)-silk fibroin-collagen nano nerve conduits.METHODS:Totally 36 female SD rats were underwent 10 mm sciatic nerve defects.The nerves of rats were grafted by reverse nerve autografts,electrostatic spinning PLGA-silk fibroin-collagen nerve conduits and PLGA nerve conduits alone.At week 12 postoperatively,nerve regeneration was studied by general observations,electrophysiological examination,microscopic observation,transmission electron microscopy observation and image analysis.RESULTS AND CONCLUSION:The results showed that the nanofibers-based nerve conduits prepared by electrostatic spinning had a well-distributed porous structure and could simulate the structure of native extracellular matrix.These findings suggest that the electrostatic spinning PLGA-silk fibroin-collagen nerve conduits group is superior to PLGA nerve conduits group in sciatic nerve regeneration,regenerative nerve myelinization and nerve foundation reconstruction,but slightly worse than the nerve autograft group.更多还原