【摘要】 背景:Runx-2是骨形态发生蛋白信号的靶目标,骨形态发生蛋白信号转导通路参与了成骨细胞对机械离心力刺激的生理响应过程,而且在成骨细胞对力学信号引起的信息传递级联反应中扮演了重要角色。目的:观察机械离心力对成骨细胞骨形态发生蛋白信号通路的影响。方法:将MC3T3-E1细胞用体积分数为10%胎牛血清DMEM培养基预处理24h后,分为对照组,90r/min组、180r/min及250r/min组,每组再分为离心1,3,6h亚组,给予不同转速和不同时间离心力刺激。对照组同步置于除离心外相同的环境中。收获细胞,提取总RNA,并反转录为cDNA,通过实时荧光定量PCR检测Runx2基因表达情况。结果与结论:随着加力时间的延长,Runx2mRNA的表达增加,二者成正相关,转速为180r/min组的Runx2mRNA表达明显高于90r/min组和250r/min组(P<0.01);90r/min组和250r/min组Runx2mRNA的表达稍高于对照组,差异无显著性意义(P=0.119)。结果可见离心力大小和离心持续时间不同对成骨细胞骨形态发生蛋白信号通路的生理响应不同,该通路在对力学信号引起的信息传递级联反应中起重要作用。更多还原

【Abstract】 BACKGROUND:Runx-2 is the target for the bone morphogenetic protein signaling;signal transduction pathway of bone morphogenetic protein involves in the physiological response process of osteoblasts to the stimulation of mechanical centrifugal force.And it plays an important role in the osteoblast information transfer cascade reactions caused by mechanical signals.OBJECTIVE:To investigate the effect of mechanical centrifugal force on the signaling pathway of osteoblast bone morphogenetic protein.METHODS:MC3T3-E1 cells were pretreated with Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum for 24 hours,and then divided into control group,90 r/min group,180 r/min group and 250 r/min group according to the rotational speed.Each group was divided into three subgroups:6 hours subgroup,12 hours subgroup and 24 hours subgroup according to the stimulation times of entrifugal force.The control group was treated in the same environment without centrifugation.The total RNA of each group was extracted,and reversed transcripted to cDNA.The expression of Runx2 was analyzed by quantitative real-time PCR.RESULTS AND CONCLUSION:With the extension of applying time,the expression of the Runx-2 mRNA increased;it has a positive correlation with the applying time.The expression of the Runx-2 mRNA in the 180 r/min group was significantly higher than that in the 90 r/min group and 250 r/min group (P < 0.05);the expression of the Runx-2 mRNA in the 90 r/min group and 250 r/min group were slightly higher than that in the control group (P=0.119).The results show that different size of centrifugal force and different duration of centrifugation has different effects on the physiological responses of osteoblast bone morphogenetic protein signaling pathway.It plays an important role in the osteoblast information transfer cascade reactions caused by mechanical signals.更多还原