【摘要】 背景:一定浓度的锶对人体健康有益,锶对人体多种细胞的增殖和酶活性有影响。目的:用不同浓度的锶矿泉水配制的培养基在体外培养人肾小管上皮细胞,观察细胞增殖情况及Na+-K+-ATP酶的活性。方法:体外培养人肾小管上皮细胞,用普通DMEM液体培养基(高糖)培养24h后,分别用含锶质量浓度2,4,6,8mg/L的矿泉水和双蒸水的条件培养基继续培养,分别于48,72,96和110h行MTT法观察HK-2细胞的增殖情况。培养110h的细胞以比色法测无机磷的量来判断Na+-K+-ATP酶的活性。结果与结论:MTT结果表明:质量浓度2mg/L锶矿泉水对HK-2细胞增殖无影响;细胞培养至110h,双蒸水组出现生长抑制时,质量浓度4,6,8mg/L锶矿泉水组却依然能保持旺盛的增殖状态(P<0.05),生长周期延长,尤以质量浓度4mg/L锶矿泉水组的效果最突出(P<0.05)。Na+-K+-ATP酶活性测试结果表明:与双蒸水组比较,锶矿泉水组Na+-K+-ATP酶活性更高(P<0.05),其中质量浓度6mg/L锶矿泉水组的酶活性最强(P<0.05)。结果证实,锶矿泉水可延长肾小管细胞的增殖周期,可促进肾小管细胞的转运功能。更多还原

【Abstract】 BACKGROUND:A certain concentration of strontium is beneficial for the human body.Strontium can affect the proliferation and enzyme activity of a variety of cells in the human body.OBJECTIVE:To investigate the effects of strontium mineral waters with different concentrations on the proliferation and Na+-K+-adenosine-triphosphatase(Na+-K+-ATPase) activity of human renal tubular epithelial cells(HK-2 cells).METHODS:Human renal tubular epithelial cells were cultured in vitro.After cultured in a normal Dulbecco’s Modified Eagle’s Medium(high glucose) for 24 hours,the cells were cultured in the conditioned medium containing 2,4,6,8 mg/L strontium mineral waters and double distilled water.The proliferation of HK-2 cells was observed by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide(MTT) assay at hours 48,72,96 and 110.The Na+-K+-ATPase activity of the cells cultured for 110 hours was judged by measuring the amount of inorganic phosphorus.RESULTS AND CONCLUSION:The results of MTT showed that 2 mg/L strontium mineral water could not affect the proliferation of HK-2 cells.When the cells were cultured for 110 hours,the cells in the double distilled water group were found growth inhibition,while the proliferation of the cells in the 6,8,10 mg/L strontium mineral water groups could still keep vigorous(P < 0.05).Moreover,the cell growth cycle was prolonged in the 6,8,10 mg/L strontium mineral water groups,especially in the 4 mg/L strontium mineral water group(P < 0.05).Compared with the double distilled water group,the Na+-K+-ATPase activity of strontium mineral waters groups was higher in the strontium mineral water groups(P < 0.05),especially in the 6 mg/L strontium mineral water group(P < 0.05).These findings suggest that the strontium mineral water with higher concentration can prolong proliferation cycle of renal tubular cells and promote the transport function of renal tubular cells.更多还原