【摘要】 背景:基于脊髓空洞常与脊柱侧凸相伴发的特点,构建具有类似临床特征的动物模型,有利于脊柱侧凸发生机制的深入研究。目的:构建具有类似临床特点的颈脊髓空洞伴脊柱侧凸兔模型。方法:将46只健康日本大耳白兔随机分组:实验组直接于第7颈椎水平脊髓内注入白陶土构建颈脊髓空洞伴脊柱侧凸模型,假手术组仅予穿刺而不推注,生理盐水组以氯化钠溶液推注。结果与结论:实验组2周见到脊髓炎症细胞浸润,4周血管间隙扩大、淋巴细胞浸润,4～6周时开始形成空洞,MRI发现颈段脊髓空洞,约67%的动物形成空洞,且随着时间延长逐渐增大,受累节段增多,组织学证实了MRI发现;X射线片出现脊柱侧凸。说明该动物模型与临床接近,具有典型的空洞及脊柱侧凸表现,且操作简单,成功率较高。更多还原

【Abstract】 BACKGROUND:Based on the characteristics that syringomyelia and scoliosis often accompany each other,building an animal model with similar clinical characteristics can facilitate the further research on the occurrence mechanism of scoliosis.OBJECTIVE:To build a rabbit model with similar clinical characteristics of cervical syringomyelia accompanied with scoliosis.METHODS:A total of 46 healthy Japanese white rabbits were randomly divided into experiment group,sham operation group and saline group.Experimental rabbits were injected with kaolin at the 7th cervical level to construct cervical syringomyelia model accompanied with scoliosis.Sham operation rabbits were punctured without injection.Rabbits in the saline group were injected with sodium chloride solution.RESULTS AND CONCLUSION:Inflammatory cell infiltration in spinal cord was found in the experiment group in the 2nd week.Vascular clearance expansion and lymphocytes infiltrate was found in the experiment group in the 4th week.Cavities started to form in 4 to 6 weeks;MRI examination showed cavities in the cervical segments.Cavities were found in about 67% of the experimental animals;the cavity size and the amount of involved segments increased with time.Histological examination confirmed the MRI results.X-ray images showed scoliosis.These findings indicate that the constructed model has typical cavities and classic scoliosis representation;it is simple to operate and has high success rate.Therefore,the constructed animal model is close to clinical cervical syringomyelia accompanied with scoliosis.更多还原