【摘要】 背景:金纳米颗粒在近红外激光照射下对肿瘤细胞具有杀伤效应。目的:评价金纳米链及anti-EGFR/Au共轭物近红外照射对人喉癌Hep-2细胞的生长抑制和热杀伤作用。方法:取对数生长期的Hep-2细胞,随机分为空白对照组、单纯照射组、金纳米链照射组和anti-EGFR/Au照射组,加入不同培养液后,各照射组进行近红外激光照射。观察照射后细胞凋亡情况和凋亡程度。结果与结论:倒置显微镜下观察显示空白对照组和单纯照射组Hep-2细胞仍具有活性,金纳米链照射组有明显的损伤,但anti-EGFR/Au照射组损伤程度更重。流式细胞分析显示金纳米链照射组及anti-EGFR/Au靶向照射组均有不同程度的Hep-2细胞凋亡,且照射时间越长,凋亡细胞越多。说明金纳米链近红外照射可以杀伤人喉鳞癌Hep-2细胞,anti-EGFR/Au靶向照射比金纳米链照射效果更好,并呈现一定照射时间的依赖性。更多还原

【Abstract】 BACKGROUND:The gold nanoparticles have a killing effect on tumor cells under the near-infrared laser irradiation.OBJECTIVE:To evaluate the growth inhibition and heat killing effect of gold nanochain and anti-epidermal growth factor receptor(EGFR)/Au conjugate on human laryngeal cancer Hep-2 cells under near-infrared hyperthermia.METHODS:The Hep-2 cells in the logarithmic growth phase were collected and divided into blank control group,irradiation group,gold nanochain irradiation group and anti-EGFR/Au irradiation group.After added with different culture medium,the near-infrared laser irradiation was performed in each group.Then the apoptosis of Hep-2 cells in different experimental groups was observed after the near-infrared laser irradiation.RESULTS AND CONCLUSION:Inverted microscope observation showed that the Hep-2 cells in the blank control and irradiation groups were still active and the Hep-2 cells in the gold nanochain irradiation group were damaged significantly,while the Hep-2 cells in anti-EGFR/Au irradiation group were damaged more serious.Flow cytometry analysis showed that the Hep-2 cells in the gold nanochain irradiation and anti-EGFR/Au irradiation groups had a varying degree of apoptosis and the increasingly heavy damage with the time.The gold nanochain irradiation can kill Hep-2 cells and the anti-EGFR/Au irradiation has a better effect in a time-depended manner.更多还原