【摘要】 背景:研究发现骨髓间充质干细胞上存在雌激素受体,雌激素通过调节骨髓间充质干细胞的增殖、分化特性发挥促成骨作用。目的:观察17β-雌二醇对骨髓间充质干细胞向成骨细胞分化的诱导作用,并探讨其作用机制。方法:在基础成骨诱导培养基培养的第3代大鼠骨髓间充质干细胞中分别加入0(对照组),0.001,0.01,0.1nmol/L17β-雌二醇干预。Elisa法检测培养的骨髓间充质干细胞分泌Ⅰ型胶原的水平,RT-PCR及Western blotting法分别检测Runx2因子mRNA及蛋白水平。结果与结论:与对照组比较,在给予0.001,0.01,0.1nmol/L17β-雌二醇干预后第5天,骨髓间充质干细胞Ⅰ型胶原表达显著升高(P<0.05),第7天仍旧呈高表达(P<0.05)。同时,在17β-雌二醇干预的第5,7天,Runx2因子mRNA及蛋白表达水平随17β-雌二醇浓度的增加而升高(P<0.05),并呈剂量依赖性。表明17β-雌二醇可诱导骨髓间充质干细胞向成骨细胞转化,其可能通过上调Runx2因子表达发挥促成骨作用。更多还原

【Abstract】 BACKGROUND:The estrogen receptor exists in bone marrow mesenchymal stem cells and estrogen promotes bone formation by regulating the proliferation and differentiation of bone marrow mesenchymal stem cells.OBJECTIVE:To explore the effects of 17β-estradiol on the osteogenic differentiation of bone marrow mesenchymal stem cells(MSCs) and the underlying mechanism.METHODS:MSCs were separated,purified,cultured and expanded.Different concentrations of 17β-estradiol was administrated to MSCs for some period.The level of type I collagen in culture medium was detected by Elisa method,and the expression of Runx2 mRNA and protein was analyzed by RT-PCR or Western blotting methods,respectively.RESULTS:Compared with the control group,type I collagen expression in the MSCs was significantly increased on the 5th day after administration of 0.001,0.01,0.1 nmol/L 17β estradiol(P < 0.05) and it maintained high expression on the 7th day(P < 0.05).Runx2 mRNA expression increased in a dose-dependent manner on the 5th and 7th days after administration of 17 β-estradiol(P < 0.05).These findings suggest that 17β-estradiol can induce the osteogenic differentiation of MSCs,which occurs possibly by upregulation of Runx2 expression.更多还原