【摘要】 目的观察白细胞介素13(IL-13)对急性缺血再灌注肾损伤大鼠IL-6表达的影响。方法将Wistar雄性大鼠37只随机分为假手术组、I/R组、C组、T-S组和T-L组。阻断大鼠双侧肾脏血流45 min,再灌注24 h建立急性肾缺血再灌注模型;T-S组和T-L组于阻断血流后分别从双侧肾动脉开口注射入鼠重组白细胞介素13 0.5μg/kg和1.5μg/kg;C组以生理盐水代替。检测各组大鼠IL-6血清水平和肾脏表达情况以及肾功能和肾脏病理变化。结果 T-L组肾脏IL-6基因和蛋白表达明显减少,IL-6血清水平也明显下降;T-L组肾功能障碍、肾组织病理变化明显减轻,肾小管损害评分明显减少;血清IL-6水平和肾组织IL-6 mRNA与BUN、Cr均呈正相关。结论 IL-13能有效抑制大鼠急性肾缺血再灌注损伤IL-6的表达和保护肾功能。更多还原

【Abstract】 Objective It is to observe the effects of IL-13 on expression of IL-6 in acute renal ischemia/reperfusion injury in rats.Methods Thirty-seven male Wistar rats were randomly divided into 5 groups: sham group,I/R group,C group,T-S group and T-L group.Models of acute renal ischemic/reperfusion injury were established by blocking up kidney’s blood flow in both side for 45 min and reperfusion for 24h in the rats.Rm-IL-13 was injected into the renal arteries through the abdominal aorta in T-S group and T-L group(T-S 0.5 μg/kg body weight,T-L 1.5 μg/kg body weight),normal saline instead of rm-IL-13 was injected into the renal arteries through the abdominal aorta in control group.The serum level of IL-6 and the renal expression of IL-6 were determined in each group at 24 h post-ischemia.In addition,BUN,Cr and renal histology were also measured.Results The serum level of IL-6 gene expression and protein production of IL-6 in kidney decreased markedly in T-L group.Renal function and histology were significantly improved in T-L group,renal injury scores decreased significantly too.A positive correlation was found between the serum level of IL-6,gene expression IL-6 in kidney and BUN,SCr.Conclusion IL-13 can inhibit the expression of IL-6 and improve function and histology of kidney in rats with acute renal ischemia/reperfusion injury.更多还原