【摘要】 <正>精子发生(spermatogenesis)是受基因调控的复杂的发育过程,精子形成不同阶段生精细胞内基因特异性表达导致顶体的发生、精核形态的建成及尾部的形成,精细胞内特有的微管套(manchette)和活更多还原

【Abstract】 Immunofluorescence and confocal microscopy were employed to investigate the localization pattern of a KIFC1-like protein and NUP62 as well as their relationship during spermiogenesis in Octopus tankahkeei.In early spermatid,the nucleus was round or oval,expression of the KIFC1-like protein was not obvious,and NUP62 was abundantly located in the nuclear membrane.In intermediate spermatid,the nucleus became spindle-shaped,the KIFC1-like protein was apparently expressed and located around the nucleus,and NUP62 was mainly located in the nuclear membrane.In late spermatid,the nucleus resembled a long spindle,the KIFC1-like protein was concentrated in proximity to the nuclear periphery and also located within the nucleus,and NUP62 was distributed in the nuclear membrane and within the nucleus.In immature sperm,the nucleus was cylindrical,distribution of the KIFC1-like protein and NUP62 was similar to that in late spermatid,and the KIFC1-like protein was enriched at two ends of the cell.In mature sperm,the nucleus became long rod-shaped,expression of the KIFC1-like protein was not detected,and expression of NUP62 was maintained at a high level.The results indicated that the KIFC1-like protein was probably involved in the biogenesis of acrosome and through its interaction with NUP62 or other mediator,associated with some macromolecular assembly in the nuclear membrane,thus providing the nucleus with direct shaping force or influencing the shaping process via indirect regulation.更多还原