【Objective】 The protein Sumoylation system was constructed in Prokaryotic cell to lay a foundation for quantity production of sumoylated protein.【Method】 Total RNA was extracted from the mouse teratoma cells F9 with Trizol reagent,reverse transcripted to cDNA,thus obtaining the genes of Sumo1,Ubc9,Sae2,Sae1 fragments by the standard PCR from cDNA.The above fragments and vectors were double digested with enzymes respectively,then they were ligated to constructed recombinate plasmids.The Sumo ylation prokaryo...
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