【摘要】 选用8个核桃无性系作为试验材料,经不同低温(4℃、-3℃、-6℃、-9℃、-12℃,4℃为对照)处理后,测定其叶片电导率、可溶性糖和丙二醛(MDA)含量,对核桃抗寒性进行研究。结果表明:随着胁迫温度的下降,叶片相对电导率呈现出"S"型上升趋势,核桃离体叶片半致死温度(LT50)在-5～12℃之间;可溶性糖与丙二醛含量的变化为先增加再下降,均在-6℃出现峰值,但抗寒性与可溶性糖含量呈正相关,而与丙二醛含量呈负相关。3种方法测定的核桃抗寒性基本一致,表明3个指标均可作为核桃抗寒性的鉴定方法。更多还原

【Abstract】 Cold resistance of 8 Juglans sigillata clones were examined by measuring the indices such as electrical conductivity,soluble sugar and malondialdehyde(MDA) contents in the leaves that were undergone different low temperatures(4℃,-3℃,-6℃,-9℃,-12℃,in which 4℃ was used as control).The results showed that with the decrease of the stress temperature,the relative electrical conductivity tended to rise as a "S" curve.The semilethal temperatures of the leaves in vitro(LT50)were between-5℃ to 12℃.Soluble sugar and MDA contents increased firstly then decreased with the decrease of temperature,two indices all arrived the maximum values at-6℃.The content of soluble sugar was positively correlated with the cold resistance,MDA however,was negatively correlated with the severeness of the coldness.The results of the cold resistance of 8 clones from the three evaluation indices were basically identical,indicating that they all could be applied in the evaluation of the cold resistance of J.sigillata.更多还原