【摘要】 目的:探讨HIV感染者外周血中CD4+CD25+Foxp3+调节性T细胞(Treg)、CD4+CD25+CD127low/-Treg的水平及其与其他免疫指标的关系。方法:采集68例未经抗HIV治疗的HIV/AIDS患者(长期不进展组即LTNP组29例、典型进展的HIV感染组27例、AIDS组12例)及20例健康成人的外周抗凝全血,经免疫荧光染色,应用流式细胞仪分析CD4+T细胞、CD8+T细胞、NK细胞及CD4+CD25+Foxp3+/CD127low/-Treg的含量,并进行统计学分析。结果:除CD8+T细胞外,HIV/AIDS患者外周血中CD4+T细胞、NK细胞、CD4+/CD8+结果均明显低于健康对照组(P<0.05);随着疾病的进展,LTNP组、HIV组、AIDS组CD4+T细胞百分比、绝对值计数,CD8+T细胞绝对计数,NK细胞绝对计数,CD4+/CD8+比值逐渐下降,而CD8+T细胞百分比逐渐上升。对CD4+CD25+Foxp3+Treg与CD4+CD25+CD127low/-Treg百分含量、绝对计数进行多重比较发现,各组间CD4+CD25+Foxp3+Treg与CD4+CD25+CD127low/-Treg所占CD4+T细胞百分含量的差异均有统计学意义(P<0.05),并且随着疾病的发展,CD4+CD25+Foxp3+/CD127low/-Treg细胞百分含量逐渐上升,LTNP组与健康对照组之间、HIV组和AIDS组之间CD4+CD25+Foxp3+/CD127low/-Treg绝对计数差异无统计学意义(P>0.05),其余各组间的差异均有统计学意义(P<0.05),并且随着疾病的发展,CD4+CD25+Foxp3+/CD127low/-Treg绝对计数逐渐下降。结论:CD4+CD25+Foxp3+/CD127low/-Treg在HIV持续感染的免疫发病机制中有一定作用。更多还原

【Abstract】 AIM: To explore the subpopulation of CD4+ CD25+ Foxp3+ regulatory T cells(Treg),CD4+ CD25+ CD127low/-Treg in peripheral blood of HIV-infected patients and study its correlation with other immune indicators.METHODS: We enrolled 68 cases of HIV/AIDS patients without anti-HIV treatment [29 cases of long-term non-progressive(LTNP) group,27 cases of typical progressive HIV infection group and 12 cases of AIDS group] and 20 healthy individuals as a control group.Blood samples of these cases were analyzed by flow cytometry after immunofluorescent staining to determine the levels of CD4+ T cells,CD8+ T cells,NK cells and CD4+ CD25+ Foxp3+/CD127low/-Treg.RESULTS: Except CD8+ T cells,the levels of CD4+ T,NK cells and CD4+/CD8+ in peripheral blood of HIV/AIDS patients were significantly lower than those in the control group(P<0.05);With the progression of disease,the percentage and absolute count of CD4+T cells,the absolute counts of CD8+T cells and NK cells,and CD4+/CD8+ T cell ratio in the LTNP group,HIV group and AIDS group decreased gradually,while the percentage of CD8+T cells increased gradually.Our multiple comparison analysis revealed that the percentages of CD4+ CD25+ Foxp3+ Treg and CD4+ CD25+ CD127low/-Treg in CD4+ T cells were significantly different among groups(P<0.05).With the progression of disease,the percentages of CD4+ CD25+ Foxp3+ Treg and CD4+ CD25+ CD127low/-Treg increased gradually;in addition,the difference in the absolute count of CD4+ CD25+ Foxp3+/CD127low/-Treg was not statistically significant between LTNP group and healthy control group(P>0.05),so was between HIV and AIDS groups(P>0.05);no significant difference was found in every other two groups(P<0.05);the absolute count of CD4+ CD25+ Foxp3+/CD127low/-Treg decreased gradually.CONCLUSION: CD4+ CD25+ Foxp3+/CD127low/-Treg may play a role in the immunopathogenesis of persistent HIV infection.更多还原