【摘要】 目的:检测体外受精-胚胎移植(IVF-ET)术后早期自然流产患者绒毛和蜕膜组织凋亡及相关调控蛋白血管内皮生长因子(VEGF)、Bcl-2的表达,探讨其发病和基因调控机制。方法:采用免疫组织化学染色技术检测IVF-ET术后早期自然流产者(IVF-ET组)绒毛及蜕膜组织Bcl-2、VEGF的表达水平,并以同期正常早孕者(正常早孕组)的标本为对照。结果:VEGF主要表达于绒毛滋养细胞和血管内皮细胞胞浆。IVF-ET组VEGF表达明显低于正常早孕组,差异具有统计学意义(P<0.05)。Bcl-2主要在绒毛合体滋养细胞上表达。IVF-ET组滋养细胞及蜕膜细胞染色较浅,表达呈弱阳性,正常早孕组染色较深,表达强阳性,差异有统计学意义(P<O.05)。结论:IVF-ET术后早期自然流产者绒毛及蜕膜组织中VEGF及Bcl-2表达显著降低,提示早期妊娠中VEGF及Bcl-2的低表达可能是导致IVF-ET术后早期自然流产的原因之一。更多还原

【Abstract】 Objective: To investigate the human chorionic villi and deciduas apoptosis and related regulatory protein(VEGF,Bcl-2) expression in early spontaneous abortion after treatment of in vitro fertilization-embryo transfer(IVF-ET) and explore the pathomechanism of spontaneous abortion.Methods: The expression of VEGF and Bcl-2 in the chorionic villi and deciduas was detected and analyzed in 30 cases of early spontaneous abortion patients after in vitro fertilization-embryo transfer(IVF-ET) through immunohistochemisty method,Thirty cases of normal early pregnancy as control.Results: Immunohistochemistry displayed that VEGF mainly expressed in cytoplasm of villi trophocyte and vascular endothelial cell.The VEGF level of early spontaneous abortion after IVF-ET group was significantly lower than that of normal early pregnancy group(P<0.05).Bcl-2 mainly expressed in the villi syncytiotrophoblast.Trophoblasts and decidual staining were shallow and weakly positive in early spontaneous abortion after IVF-ET group.But trophoblasts and decidual of normal early pregnancy group were deeply stained,the expression was strong positive.Difference between two groups was significant(P<0.05).Conclusion: The expression of VEGF and Bcl-2 decreases significantly in both chorionic villi and deciduas of early spontaneous abortion after IVF-ET.We speculate that the reduction of the expression of VEGF and Bcl-2 in the deciduas and chorionic villi in early gestation stage may cause abortion.更多还原