【摘要】 背景:胃动素（MTL）和生长抑素（SST）是对胃肠动力有兴奋和抑制作用的脑肠肽,在胃肠动力障碍中起重要作用。目的:研究脊髓损伤大鼠胃肠动力的变化及其可能的作用机制。方法:将138只大鼠随机分为模型组和对照组,采用重物坠落法建立脊髓损伤模型。造模后第1 d、7 d分别处死大鼠,行HE染色观察脊髓组织,并检测胃排空和小肠推进率。采用RT-PCR法检测MTL-R1A和SSTR₂mRNA表达。结果:造模后第1 d、7 d,模型组脊髓组织形态学发生明显变化。造模第7 d,与对照组相比,模型组胃内核素残留率明显增加（76.66%±11.84%对55.32%±10.88%,P<0.01）,小肠推进率明显减少（31.64%±5.47%对43.56%±7.00%,P<0.01）;MTL-R1A mRNA表达明显降低（0.21±0.07对0.47±0.13,P<0.01）,SSTR2mRNA表达明显升高（1.12±0.21对0.62±0.13,P<0.01）。结论:脊髓损伤后胃肠动力障碍的发生可能与胃组织MTL-R1A mRNA表达下调以及结肠组织SSTR₂mRNA表达上调有关。更多还原

【Abstract】 Background:Motilin（MTL） and somatostatin（SST） are the brain-gut peptides that have excitatory or inhibitory effect on gastrointestinal motility,respectively,and play an important role in gastrointestinal motility disorder.Aims:To study the change of gastrointestinal motility and possible mechanism in rats with spinal cord injury.Methods:One hundred and thirtyeight rats were randomly divided into model group and control group.The spinal cord injury model was established by weight drop method.Rats were sacrificed on the 1^st,7^th day after establishment,HE staining was used to observe spinal cord tissue injury,while gastric emptying and propulsion rate of small intestine were measured.Expressions of MTL-RIA mRNA and SSTR₂ mRNA were determined by RT-PCR.Results:On the 1^st 7^th day after establishment,morphology of spinal cord tissue in model group obviously changed.On the 7^th day after establishment and compared with control group,gastric nuclide remnant rate in model group was significandy increased（76.66%±11.84%vs.55.32%±10.88%,P <0.01）,propulsion rate of small intestine was significantly decreased（31.64%±5.47%vs.43.56%±7.00%,P <0.01）;expression of MTLR1A mRNA in model group was obviously decreased（0.21±0.07 vs.0.47±0.13,P <0.01）,while expression of SSTR₂ mRNA was significantly increased（1.12±0.21 vs.0.62±0.13,P < 0.01 ）.Conclusions:The occurrence of gastrointestinal motility disorder after spinal cord injury may be related to the down-regulation of expression of MTL-RIA mRNA in gastric tissue and up-regulation of expression of SSTR₂ mRNA in colonic tissue.更多还原