【摘要】 目的探讨原位肝移植(OLT)术后乙肝复发患者肝内免疫活性细胞与乙肝病毒(HBV)慢性感染的关系。方法运用免疫组织化学法回顾性分析12例OLT术后乙肝复发患者肝内CD3+、CD4+、CD8+以及CD4+CD25+调节性T细胞(Treg)的表达,并与12例慢性乙肝(CHB)患者进行比较。结果淋巴细胞浸润主要集中在汇管区,OLT术后乙肝复发患者肝脏浸润的CD3+、CD4+、CD8+T细胞数量显著低于CHB患者,Treg明显升高,差异均具有统计学意义(P<0.05)。Treg细胞随着炎症分级(G)的升高而增多;高病毒载量组明显高于低病毒载量组且免疫组化HBsAg、HBeAg阳性增强。结论 OLT术后乙肝复发患者机体呈现免疫抑制状态,病理炎症反应轻于CHB患者。肝组织中Treg细胞在免疫抑制、控制肝脏炎症反应、病毒复制等方面可能起了重要作用。更多还原

【Abstract】 Objective To explore the relationship between intrahepatic immune competent cells and the status of chronic HBV infection in patients with HBV recurrence after orthotopic liver transplantation(OLT).Methods 12 liver biopsies,collected from HBV recurrent patients after OLT were detected by immunohistochemistry for the expression of intrahepatic CD3+,CD4+,CD8+,CD4+CD25+ regulatory T cell(Treg) T lymphocytes,and compared with 12 chronic hepatitis B(CHB) liver biopsies.Results Lymphocyte infiltrated mainly concentrated in the portal area.The number of CD3+,CD4+,CD8+ T cells were reduced and the number of Treg were increased significantly in HBV recurrent patients after OLT compared with CHB(P<0.05).The expression of Treg were associated with pathology grade.The high expression of Treg was associated with the high viral load and the positive immunohistochemical expression of HBsAg,HBeAg were enhanced.Conclusion Immunosuppression exists in HBV recurrent patients after OLT and pathological damage was lighter than CHB.Intrahepatic Treg may play important roles in immune suppression,and control of liver inflammation and viral replication.更多还原