【摘要】 考察酶法水解VC磷酸酯镁(Magnesium Ascorbyl Phosphate,MAP)过程,采用复合磷酸酯酶(Phosphoesterase Complex,PC)为催化剂,以水解产物VC的含量(水解率)为指标考察底物浓度、反应时间、pH值、反应温度、酶用量等因素对MAP水解程度的影响,选择最佳水解条件并对其进行动力学分析。结果表明,在底物MAP的质量浓度为1.72g/L、酶和底物的体积分数为5%、温度37℃、pH 5.5条件下水解4h,水解率最大,可达(32.64±0.42)%。动力学分析表明:水解动力学符合米氏方程,其中km=25.82g/L,vmax=126.26g/(L.h)。更多还原

【Abstract】 In order to investigate the process of enzymatic hydrolysis of magnesium ascorbyl phosphate(MAP),the best hydrolysis condition was chosen and the kinetics was analyzed through the experiments.With phosphoesterase complex(PC) as catalyst,the effect on the degree of MAP hydrolysis by some influenced factors such as substrate concentration,reaction time,pH,temperature,enzyme content was investigated and the hydrolysis kinetics was studied.The results show that hydrolysis degree of MAP reached the largest,to(32.64±0.42) % under the conditions of 1.72g/L substrate concentration by 5% enzyme,37 ℃,pH 5.5,hydrolysis time 4h.The established kinetics followed the equation of Michealis-Menten,km and vmax of which were 25.82 g/L and 126.26 g/(L·h),respectively.更多还原