【摘要】 从天津大港油田PAHs污染的盐碱土壤中分离到1株以菲、芘为唯一碳源的真菌TJF1,经形态观察为青霉属(Penicillium)。菌株TJF1在含菲、芘浓度分别为25、50和75 mg L-1的液体无机盐培养基中培养15 d降解率为75.7%和50.1%、41.3%和27.9%、23.9%和12.5%。菌株TJF1对菲、芘的降解具有较广泛的pH和盐度范围,在菲、芘总浓度100 mg L-1,pH5.3~9.5,盐度0%~10%的液体条件下均可生长及有较高的降解效果,为了提高其对菲、芘的降解效率,在底物中添加一定量的葡萄糖,结果表明:葡萄糖对菲、芘降解率影响呈抛物线状,葡萄糖浓度在0.8%时,TJF1对菲、芘的降解率达到63.8%和57.2%。更多还原

【Abstract】 The predominant indigenous microbial population and the strain TJF1 PAHs-degradation were developed by selective enrichment culture from oil-contaminated saline soil in Dagang Oilfield.Morphology analysis revealed that strain TJF1 belonged to Penicillium.When PAHs initial concentration was 25 mg L-1,500 mg L-1 and 75 mg L-1,the degradation rates of phenanthrene and pyrene by TJF1 were separately 75.7% and 50.1%,41.3% and 27.9%,23.9% and 12.5%.Phenanthrene and pyrene could be biodegraded at different pH values ranging from 5.3 to 9.5 and different salinity ranging from 0% to 5% in the medium contains 100 mg L-1 phenanthrene and pyrene.Our results indicated that more than 63.8% of phenanthrene and 53.2% pyrene were degraded in the medium containing 0.8% glucose.更多还原