【摘要】 以马铃薯葡萄糖为培养基进行猴头菌液体静置发酵。利用萃取、中压液相色谱(RP-18 gel)、凝胶柱层析(Sephadex LH-20)和硅胶柱层析(Silica gel)从其发酵液中分离并结构鉴定了6个化合物。经核磁共振波谱解析与质谱鉴定,确定化合物的结构为Erinacine P(1)、Orsellinaldehyde(2)、Herierin IV(3)、Erinapyrone A(4)、Erinapyrone B(5)和Erinapyrone C(6)。Erinacine P(1)为首次从猴头菌发酵液中分离获得的二萜糖苷化合物,MTT实验表明其具有较强的细胞毒活性。Orsellinaldehyde(2)为首次从猴头菌中分离到的苯甲醛衍生物。Herierin IV(3)、Erinapyrone A(4)、Erinapyrone B(5)和Erinapyrone C(6)为4个吡喃酮衍生物。更多还原

【Abstract】 Hericium erinaceus was cultured by surface liquid fermentation with Potato-Dextose medium.The fermented solution was extracted and purified by repeated column chromatography(RP-18,Sephadex LH-20 and silica gel) to afford six chemical components.Based on the NMR and ESI MS data,the structures of the six components were elucidated as erinacine P(1),orsellinaldehyde(2),herierin IV(3),erinapyrone A(4),erinapyrone B(5) and erinapyrone C(6).Erinacine P(1) was isolated from the fermented solution of H.erinaceus for the first time and it showed high bioactivity in the MTT assay against HeLa cells.Orsellinaldehyde(2),named 2,4-dihydroxy-6-methylbenzaldehyde,was separated from H.erinaceus for the first time.Herierin IV(3),erinapyrone A(4),erinapyrone B(5) and erinapyrone C(6) were all the pyrone derivatives.更多还原