【摘要】 [目的]探讨PESV对K562细胞PI3K/Akt信号蛋白及凋亡调控因子Bcl-2和Bad表达的影响。[方法]将体外培养K562细胞,经PESV处理不同时间后,流式细胞术检测细胞凋亡率,Western blot检测PI3K及p-Akt蛋白水平变化,实时荧光定量RT-PCR检测Bcl-2、Bad mRNA水平变化。[结果]与对照组相比,PESV处理后K562细胞凋亡率显著增加,PI3K及p-Akt表达降低,抗凋亡相关基因Bcl-2 mRNA表达降低,促凋亡基因Bad mRNA表达增加。[结论]PESV可能通过降低PI3K、Akt信号蛋白表达,调节Bcl-2和Bad表达,抑制K562细胞增殖,促进其凋亡。更多还原

【Abstract】 [Objective] To investigate the effect of PESV on the expression of massage protein PI3K / Akt,apoptosis regulators Bcl-2 and Bad expression in K562 cells.[Methods] In vitro cultured K562 cells preconditioned with PESV for different times were determined for cell apoptosis rate with cytoflowmeter,the level of PI3K and p-AKT protein with Western blot and Bcl-2,Bad mRNA level by realtime fluorescence quantitative RT-PCR.[Results] Compared with the control group,the apoptosis rate of PESV treated K562 cells was significantly increaseds,the expression of PI3K and p-Akt and anti-apoptosis-related gene Bcl-2 mRNA was decreased;the expression of pro-apoptotic gene Bad mRNA was increased.[Conclusion] PESV may regulate the expression of Bcl-2 and Bad,inhibit the proliferation of K562 cells and promote their apoptosis by reducing the expression of PI3K and Akt signaling protein.更多还原