【摘要】 目的通过观察活性维生素D3[1,25-(OH)2VitD3,活性VitD3]对单侧输尿管结扎(unilateral ureteralobstruction,UUO)的CD-1小鼠肾组织胶原Ⅳ蛋白(collagenⅣ)表达的研究,探讨活性VitD3阻止肾组织纤维化作用的机制。方法 42只CD-1小鼠随机分成假手术组(n=6)、UUO组(n=18)、UUO加活性VitD3治疗组(n=18),治疗组再分为0.5、0.25、1.0μg/kg 3个剂量组。UUO组分别于术后(建模后)第3、7、14天处死,其余各组均在术后第7天处死。Western印迹法检测肾脏中胶原Ⅳ蛋白表达量。结果术后第3、7、14天,UUO组肾组织胶原Ⅳ蛋白表达水平都显著低于假手术组,尤其是术后第7天,几乎检测不到胶原Ⅳ蛋白表达。UUO加活性VitD3治疗组的胶原Ⅳ蛋白表达水平显著高于UUO组(P<0.05)。结论活性VitD3对UUO所致CD-1小鼠的肾间质纤维化模型胶原Ⅳ蛋白的表达有一定的促进作用,提示活性VitD3可能具有帮助维持肾小管基底膜完整性的作用。更多还原

【Abstract】 Objective To investigate the effect of 1,25-(OH)2VitD3 on renal fibrosis in unilateral ureteral obstruction(UUO) of mice.Methods Forty-two male CD-1 mice were randomly assigned to three groups: Sham-operated group(n=6),UUO group(n=18) and UUO plus 1,25-(OH)2VitD3(0.5,0.25,1.0 μg/kg) group(n=18).Mice of UUO group were sacrificed 3,7,14 d after operation,other groups were sacrificed 7d after operation.The expression of collagen Ⅳ in renal tissues was tested by Western blotting.Results Western blotting demonstrated a significant reduction of collagen Ⅳ production in UUO group,especially at d7 following UUO;the contents of collagen Ⅳ in renal tissue of 1,25-(OH)2VitD3 group were increased compared to UUO group.Conclusion 1,25-(OH)2VitD3 ameliorates renal interstitial fibrosis in the obstructed kidneys,which is associated with the restoration of collagen Ⅳ production.更多还原