【摘要】 建立测定人参强力胶囊中6种人参皂苷(Rg1、Re、Rb1、Rc、Rb2、Rd)含量的HPLC方法。色谱柱为Alltima C18(250mm×4.6mm,5μm)柱,流动相为乙腈-0.02%磷酸水溶液,流速为1.0mL/min;梯度洗脱;检测波长为203nm;人参皂苷Rg1、Re、Rb1、Rc、Rb2、Rd线性范围分别为0.396 8～7.936μg/mL、0.406 4～8.128μg/mL、0.196 0～3.920μg/mL、0.204 0～4.080μg/mL、0.200 0～4.000μg/mL、0.201 6～4.032μg/mL,相关系数均大于0.999 0。6种人参皂苷的平均加样回收率在100.1%～103.4%之间,RSD均小于1.5%。本方法简便、快捷、准确,可以用于人参强力胶囊的质量控制。更多还原

【Abstract】 To develop an HPLC method for determination of six active ginsenosides(Rg1、Re、Rb1、Rc、Rb2、Rd)in Renshen Qiangli Capsules.Chromatographic separation was performed on an Alltima C18(250mm×4.6mm,5μm)column;mobile phase was acetonitrile-0.02% phosphoric acid,flow rate was 1.0mL/min;gradient elution;detector wavelength was 203nm.Good linear relationships of six ginsenosides were provided over investigated concentration ranges.The linear relationships of six ginsenosides Rg1,Re,Rb1,Rc,Rb2,Rd were 0.396 8-7.936μg/mL,0.406 4-8.128μg/mL,0.196 0-3.920μg/mL,0.204 0-4.080μg/mL,0.200 0-4.000μg/mL,0.201 6-4.032μg/mL,respectively.The values of correlation coefficient were higher than 0.999 0 for all the analytes.The average recoveries of the six components ranged from 100.1% to 103.4%.Repeatability experiments showed that relative standard deviation(RSD)values of the six contents were less than 1.5%.The developed method was simple,sensitive and repeatable,and can be used for quality control of Renshen Qiangli Capsules.更多还原