【摘要】 目的观察束缚-浸水应激(RWIS)对石榴皮多酚在大鼠主要器官内分布的影响。方法利用FeCl3与多酚反应显色、与梯度多酚构成色阶,比色法检测多酚在器官内的含量。Wistar雄性大鼠250～300 g,灌胃给药,随机分为对照Ⅰ组(2 ml去离子水+应激,n=5);对照Ⅱ组(500 mg/ml石榴皮水提物、未应激)和实验Ⅰ、Ⅱ、Ⅲ(分别为5,50,500 mg/ml石榴皮水提物+应激)3个剂量组。给药1 h后行RWIS 3 h。过量麻醉处死动物后,心脏灌流除血,取脑、心、胃、肺、肝、肾、回肠、结肠匀浆,离心,取上清液与FeCl3反应。结果检测石榴皮多酚的最佳FeCl3浓度为9%。呈色反应的石榴皮水提物浓度为0.2～0.5 mg/ml。在大鼠的脑、心、肺、肝、肾上清液中未观察到呈色反应。实验Ⅲ组胃、结肠上清液与FeCl3反应阳性;而对照Ⅱ组的回肠、结肠也呈阳性,且高于实验Ⅲ组。结论 9%FeCl3可检测出0.2～0.5 mg/ml石榴皮水提物中的多酚。石榴皮多酚在胃肠壁中分布多于其它器官组织。RWIS使大鼠胃壁中石榴皮多酚含量增多,回肠和结肠壁内含量减少。更多还原

【Abstract】 Objective To observe the effect of restraint and water-immersion stress(RWIS) on the distribution of pomegranate peel polyphenol(PPP)in the major organs of rats. Methods The polyphenol content in rat organ was determined by the colorimetric method.Wistar male rats were randomly divided into control group Ⅰ(2ml deionized water + RWIS,n=5),control group Ⅱ(500 mg/ml Aqueous extraction of pomegranate peel(AEPP),without stress)and experimental groups Ⅰ、Ⅱ、Ⅲ(5、50、500 mg/ml AEPP + RWIS).1 h after drug administration,the rats received RWIS for 3 hours,then the rats were killed by an overdose of onesthesia and brain,heart,stomach,lung,liver,kidney,ileum and colon were harvested,homogenate,centrifuged,and the supernatant was mixed with ferric chloride,color-reaction was observed. Results 9% ferric chloride was the best conatin for detection of PPP of AEPP.The response was obvious with AEPP 0.2～0.5 mg/ml.The supernate of brain,heart,lungs,liver,kidney had no color reaction.The supernates of stomach,colon were positive reaction with ferric chloride in experimental group Ⅲ;in the control group Ⅱileum,colon also showed positive reaction,which was higher than that in the experimental group Ⅲ. Conclusion 9% ferric chloride can sensitively detect content of PPP in 0.2～0.5 mg/ml AEPP.The PPP in gastrointestinal wall is distributed more than in other organs.RWIS is contribute to PPP enrichment at stomach wall,but reduce the PPP content at ileum wall and colon wall.更多还原