【摘要】 目的研究毛菊苣不同提取物对四氯化碳(CCl4)致小鼠急性化学性肝损伤的保护作用。方法昆明种小鼠468只,雌雄各半,随机分为正常对照组、模型组、水飞蓟宾阳性对照组(47 mg·kg-1)、毛菊苣种子提取物Ⅰ低、中、高剂量组(8,12,16 mg·kg-1)、提取物Ⅱ低、中、高剂量组(2,3,4 mg·kg-1)、提取物Ⅲ低、中、高剂量组(8,11,14 mg·kg-1)、提取物Ⅳ低、中、高剂量组(70,105,140 mg·kg-1)和提取物Ⅴ低、中、高剂量组(30,45,60 mg·kg-1)、毛菊苣全草提取物Ⅵ低、中、高剂量组(30,45,60 mg·kg-1)、提取物Ⅶ低、中、高剂量组(7,11,15 mg·kg-1)、提取物Ⅷ低、中、高剂量组(11,17,23mg·kg-1)、提取物Ⅸ低、中、高剂量组(130,195,260 mg·kg-1)和提取物Ⅹ低、中、高剂量组(170,260,350 mg·kg-1)。口服10 ml·kg-1的0.2%CCl4橄榄油溶液造极性肝损伤模型,观察各提取物对小鼠血清丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)活性的影响,并观察肝脏病理组织学变化。结果对CCl4引起的肝损伤动物模型,毛菊苣种子和全草各提取物各剂量组均能不同程度地减低小鼠血清ALT和AST活性(P<0.05或P<0.01),并能不同程度地改善肝脏病理组织损伤,其中种子和全草的乙酸乙酯部位和正丁醇部位显示较强的肝保护作用。结论亲脂性成分和亲水性成分均为毛菊苣肝保护作用的有效成分,该药材具有多成分综合作用的特点,进一步深入阐明其保肝作用化学成分意义重大。更多还原

【Abstract】 Objective To study the hepatoprotective effects of Cichorium glandulosum extracts from seeds and herbs on liver injury induced by carbon tetrachloride(CCl4) in mice. Methods 468 Kunming mice were randomly divided into normal control group,model group,positive control group(47 mg·kg-1).And low,middle,high dose groups of extract Ⅰ(8,12,16 mg·kg-1),extract Ⅱ(2,3,4 mg·kg-1),extract Ⅲ(8,11,14 mg·kg-1),Ⅳ(70,105,140 mg·kg-1),Ⅴ(30,45,60 mg·kg-1),Ⅵ(30,45,60 mg·kg-1),Ⅶ(7,11,15 mg·kg-1),Ⅷ(11,17,23 mg·kg-1),Ⅸ(130,195,260 mg·kg-1) and Ⅹ(170,260,350 mg·kg-1),respectively.The mice were given intragastrisally 0.2% CCl4 solution(10 ml·kg-1).The activities of serum ALT and AST,as well as the pathological changes of the liver were determined in all groups. Results Compared with the model group,all dose groups of the extracts could reduce serum ALT and AST activities significantly(P < 0.05 or P < 0.01).Histopathological observations showed that all extracts could ameliorate the liver injury in different degrees.Among them,ethyl acetate extract and butanol extract exhibited much higher hepatoprotective effects. Conclusion Either hydrophilic or lipophilic components in C.glandulosum showed hepatoprotective effects,which displayed multi components with comprehensive effects of Uyghur drugs.Chemical components and their hepatoprotective effects need further study.更多还原