【摘要】 目的探讨山茱萸水提液体外对D-半乳糖致衰人胚肺成纤维细胞(MRC-5)的影响作用及机制。方法 MTT法观察山茱萸水提液对D-半乳糖致衰MRC-5细胞生长状况的影响;Western blot法观察山茱萸水提液对D-半乳糖致衰MRC-5细胞抗衰老通路P16,P21的影响;Telomere PNA Kit/FITC for Flow Cytometry法观察山茱萸水提液对MRC-5细胞端粒长度的影响。结果 MTT检测山茱萸水提液对D-半乳糖致衰MRC-5细胞有促进增殖作用;Western blot法检测,与对照组比较山茱萸水提液能够使D-半乳糖致衰MRC-5细胞P16、P21表达降低;与对照组比较山茱萸水提液能够延缓端粒缩短。结论山茱萸水提液有抗衰老作用。更多还原

【Abstract】 Objective To study the effects and mechanisms of Cornus water extraction in human embryonic lung fibroblast cells(MRC-5) induced aging by D-galactose.Methods To observe growth conditions by MTT method about the water extraction of Cornus on human embryonic lung fibroblast cells(MRC-5) induced aging by D-galactose;To detect anti-aging pathway of P16,P21 in Western blot about Cornus water extraction on MRC-5 cells induced aging by D-galactose.In experiment of Telomere PNA Kit / FITC for Flow Cytometry,study telomere length influences of Cornus water extraction on MRC-5 cell.Results The results of MTT method showed the water extraction of Cornus can promote reproduce of human embryonic lung fibroblast cells(MRC-5) induced aging by D-galactose;Western blot method and Telomere PNA Kit/FITC for Flow Cytometry results reveal,compared with the control group,Cornus water extraction could make P16 and P21 expression reduced of MRC-5cell induced aging by D-galactose;and delay the telomere shortening of MRC-5 cell.Conclusion Cornus water extraction have anti-aging function.更多还原