【摘要】 目的:探讨纤溶酶原激活物抑制物-1(plasminogen activator inhibitor-1,PAI-1)抗原水平及其基因启动子-844G→A多态性与不稳定心绞痛(UAP)的关系。方法:UAP患者121例(UAP组)和非冠心病者108例(对照组),以ELISA法测定PAI-1抗原水平。PCR扩增特定DNA片段,XhoI酶切、琼脂糖电泳确定PAI-1基因启动子-844位点基因型。结果:UAP组PAI-1抗原水平(68.15±45.29)μg/L显著高于对照组(53.39±20.62)μg/L,P<0.001。PAI-1抗原水平与血糖、总胆固醇(TC)、低密度脂蛋白胆固醇(LDLc)水平正相关,r分别为0.147、0.151、0.144(P<0.05)。A、G等位基因频率为0.40、0.60,AA、AG、GG三种基因型在两组间分布差异无统计学意义,A等位基因与较高的PAI-1抗原水平有关。结论:高PAI-1抗原水平是UAP发生的危险因素,PAI-1基因启动子-844G→A基因多态性与UAP发病无关,但A等位基因携带者存在较高的PAI-1抗原水平。更多还原

【Abstract】 Objective To investigate the relatione relationship between level of PAI-1 antigen,its geng promoter-844G→A polymorphism and unstable angina pectoris.Methods 121 patients with unstable angina pectoris(UAP group)and 108 patients with angiography-proved no stenosis(control group)were studied.The level of PAI-1 antigen was measured by ELISA.The specific DNA segment of PAI-1 was amplified by PCR,and incised by endonuclease XhoI.The genotype of PAI-1 gene promoter-844 site was confirmed by Agarose Electrophoresis. Results The level of PAI-1 antigen in UAP group was significantly higher than that in control group(68.15±45.29) μg/L vs(53.39±20.62)μg/L,P<0.001).The level of PAI-1 antigen showed a positive correlation with Glu,TC and LDL(r=0.147,0.15,0.144,P<0.05).Frequency of A,G allele is 0.4,0.6,there was no important difference in genotype frequency of AA,AG,GG in both groups.A allele is associated with higher level of PAI-1 antigen. Conclusion High level of PAI-1 antigen is a risk factor for UAP.PAI-1 gene Promoter-844G→A polymorphism has no relationship with the occurrence of UAP,but there is higher level of PAI-1 antigen in carriers with A allele.更多还原