【摘要】 目的:探讨胶质细胞源性神经营养因子(GDNF)基因修饰的骨髓间充质干细胞(BMSCs)在脑出血大鼠脑内的存活与分化。方法:分别将BMSCs/GDNF、BMSCs和生理盐水移植入脑出血大鼠脑内建立不同的实验组,采用Western blot检测各组大鼠GDNF蛋白的表达,DAPI和免疫荧光组织化学染色观察移植细胞在脑内的存活和分化情况。结果:与生理盐水组和BMSCs组相比,BMSCs/GDNF组的GDNF蛋白表达显著上调;与BMSCs组相比,BMSCs/GDNF组的BMSCs存活率增高,神经微丝阳性细胞率上升,但胶质纤维酸性蛋白阳性细胞率无明显变化。结论:GDNF基因修饰有助于BMSCs在脑出血大鼠脑内的存活和向神经元样细胞方向分化。更多还原

【Abstract】 Objetive To explore the survival and differentiation of GDNF gene-modified BMSCs in a rat model of intracerebral hemorrhage(ICH).Methods Different groups were established by transplanting with BMSCs/GDNF,BMSCs and saline into the brains of ICH rats,respectively.The expression of GDNF protein was tested by western blot analysis.DAPI and immunohistochemistry staining were used to observe the survival and differentiation of transplanted cells in rat brains.Results The expression of GDNF protein was significantly increased in BMSCs/GDNF group when compared with saline group and BMSCs group.The survival rate of BMSCs increased in BMSCs/GDNF group when compared with BMSCs group.The percentage of NF-200 positive cells in BMSCs/GDNF group was more than that in BMSCs group.Conclusion GDNF gene modification of BMSCs may contribute to the survival and neuron-like differentiation of the transplanted BMSCs in a rat model of intracerebral hemorrhage.更多还原