【摘要】 目的建立一种反转录实时荧光定量PCR检测HPV mRNA表达量的方法,研究HPV mRNA表达量与宫颈癌发展的关系。方法取155例宫颈组织标本进行病理学诊断,同时分别进行HPV DNA实时荧光定量PCR检测、HPV E7 mRNA反转录实时荧光定量PCR检测。结果在所有的HPV DNA阳性标本中,宫颈炎和CINⅠ期标本mR-NA表达率为0,CINⅡ期标本mRNA表达率为50%,CINⅢ期标本mRNA表达率为50%,宫颈癌标本mRNA表达率为87.5%;表达水平依次增加。结论 HPV mRNA的表达率及表达量随宫颈癌的发展而增加和增强,其表达水平与宫颈癌发展呈正相关性;HPV mRNA定量检测方法的建立,可以为宫颈癌的预防、发展监测、手术预后等提供重要的临床指导。更多还原

【Abstract】 Objective To establish a real-time quantitative reverse transcriptionPCR for detecting HPV mRNA expression,and to study the relationship between the quantity of HPV mRNA expression and the development of cervical cancer. Methods A total of 155 cervical biopsy specimens were taken for pathological diagnosis,at the same time,HPV DNA was detected by real-time quantitative PCR and HPV E7 mRNA expression was determined by real-time quantitative reverse transcription-PCR. Results Among all HPV DNA positive samples,mRNA expression in cervicitis and CIN I period specimens was both 0,while those in CINⅡ period specimens,CIN Ⅲ period specimens,and cervical cancer specimens were 50%,50%,and 87.5%,respectively.The expression levels increased in turn. Conclusions The expression rate and expression level of HPV mRNA are increased with the development of cervical cancer,and moreover,its expression level is positively correlated with the development of cervical cancer.The establishment of HPV mRNA quantitative detection method can provide an important clinical guidance for prevention,development monitoring and postoperative prognosis of cervical cancer.更多还原