【摘要】 采用激光诱导技术(LIPSS)在聚对苯二甲酸乙二醇酯(PET)材料表面制备周期性的纳米沟槽结构。以人脐静脉血管内皮细胞(HUVEC)为细胞模型,研究蛋白吸附、溶血和凝血过程以及内皮细胞对纳米结构的响应。实验结果表明,表面纳米沟槽结构促进牛血清白蛋白吸附和聚集,减少血小板凝结,溶血率小于5%,抗凝血的BCI值随时间下降缓慢,始终保持在70-80%。此外,PET表面纳米沟槽结构有助于模拟体内血管表面,提高血管内皮细胞迁移能力,促使细胞在剪切力作用下发生规则排列且形态更趋于体内状态。由此可见,纳米沟槽的PET薄膜因有效提高其表面亲水性能,从而改善其血液相容性和内皮细胞生长状态,可作为人工血管医用材料选择之一。牛血清白蛋白在纳米沟槽和光滑的PET薄膜表面吸附的AFM结果显示,白蛋白更易吸附于纳米沟槽的PET薄膜,减少了血小板的粘附,PET材料的血液相容性得以改善。吸附溶血试验、体外动态凝血时间试验和血小板粘附SEM结果可以看出,采用纳米沟槽的PET薄膜可有效提高其表面亲水性能,改善溶血率(小于5%)和抗凝血性能。HUVEC细胞在纳米沟槽PET基底上生长时,可促进细胞粘附、增殖和迁移,并有助于细胞在受剪切力作用下规则排列,使细胞形态更趋于体内状态。更多还原

【Abstract】 In this work,the nanogrooved surface,which was fabricated on polyethylene terephthalate(PET) film by laser induced periodic structures(LIPSS) technique,was used as a model system to study blood compatibility,such as protein absorption,hemolysis rate and platelet adhesion.The human umbilical vein endothelial cells(HUVEC) were chosen as the cell model.The AFM results showed that there were more adsorbed bovine serum albumins(BSA) on the nanogrooved PET surface than on the smooth PET.The hemolysis,kinetic thrombus time and platelet adhesion tests indicated that nanostructures were helpful to improve the blood compatibility of PET films.The hemolysis rate is lower than 5%.With the increase of treat time on nanogrooved PET surface,the decrease of BCI value is very slow,between 70 and 80%.The nanogrooves were also helpful for endothelialization of PET and directed the alignment of vascular endothelial cells under shear stress.更多还原