【摘要】 目的了解紫外线照射对多药耐药鲍曼不动杆菌的杀灭效果。方法将"干燥"环境下和接种于营养琼脂培养基上的多药耐药鲍曼不动杆菌,分别置于紫外线灯下0.6 m、1 m、2 m,照射不同时间,观察杀灭效果。结果在"干燥"环境条件下多药耐药鲍曼不动杆菌于紫外线灯下0.6 m照射8 min,于紫外线灯下1 m、2 m照射120 min和30 min后,倾注营养琼脂,35℃培养48 h均无菌生长;细菌直接接种于营养琼脂培养基上,紫外线灯下0.6 m照射49 min无菌生长;紫外线灯下1 m、2 m照射30 min和120 min均有菌生长。结论 "干燥"环境下,常规的紫外线消毒方法可杀灭多药耐药鲍曼不动杆菌;高湿环境下,常规紫外线消毒方法不能完全杀灭多药耐药鲍曼不动杆菌,即使延长照射时间也不能达到理想的杀菌效果;只有缩短照射距离、延长紫外线照射时间才可以完全杀灭细菌。更多还原

【Abstract】 Objective To investigate the killing effect of ultraviolet irradiation on multi-drug resistant Acinetobacter baumannii.Methods In the "dry" environment,Acinetobacter baumannii was inoculated on nutrient agar medium with multi-drug resistant,and placed in UV lamp 0.6 m,1 m,2 m,irradiation at different times to observe the killing effect.Results In the "dry" environmental conditions,Acinetobacter baumannii was exposured to UV lamp 0.6 m for 8 min,the ultraviolet light 1 m,2 m light for 120 min and 30 min,poured nutrient agar,35 ℃ 48 h culture sterile growth;direct inoculation of bacteria in nutrient agar medium,ultraviolet light irradiation 49 min 0.6 m sterile growth;ultraviolet light 1 m,2 m 30 min and 120 min,there existed bacteria growth.Conclusion In the "dry" environment,the conventional method of ultraviolet disinfection can kill multi-drug resistant Acinetobacter baumannii;in the humid environment,conventional UV disinfection methods can not completely kill multi-drug resistant Acinetobacter baumannii,even if the extension exposure time can not achieve the desired sterilization;only shorten the distance of irradiation and extend the time before UV irradiation can completely kill the bacteria.更多还原