【摘要】 目的探讨妊娠糖尿病(GDM)患者血清及胎盘中内脂素(visfatin)的表达水平。方法采用酶联免疫吸附法及放免法分别测定30例妊娠糖尿病(GDM组)及30例正常孕妇(对照组)血清内脂素及胰岛素水平,计算胰岛素抵抗指数(HOMA-IR)及体质量指数(BMI)。采用逆转录-聚合酶链反应(RT-PCR)及蛋白印迹法,检测两组胎盘内脂素基因mRNA的表达及蛋白的表达水平。结果①GDM组血清visfatin、FBG、FIN、HOMA-IR、BMI水平显著高于对照组(P<0.05)。②GDM组胎盘组织中visfatin mRNA及蛋白表达水平显著高于对照组(P<0.05)。③血清内脂素水平与HOMA-IR呈正相关(P<0.05)。结论 GDM孕妇血清及胎盘内脂素水平的升高可能参与了GDM的发生发展,血清内脂素与胰岛素抵抗相关。更多还原

【Abstract】 Objective To investigate the expression level of visfatin in placenta and serum of gestational diabetes mellitus(GDM).Methods The levels of serum visfatin,fasting blood glucose(FBG)and fasting insulin(FIN)were measured.The insulin resistance index(HOMA-IR)was calculated.The expression of visfatin mRNA and protein were separately determined by reverse transcription polymerase chain reaction(RT-PCR)and western-blot from 30 pregnant women with GDM(GDM group)and 30 pregnant women with normal glucose(control group).Results ①The serum levels of visfatin,FBG,FIN,BMI and HOMA-IR were significantly higher in GDM group than those in the control group(P<0.05).②The levels of visfatin mRNA and visfatin protein expression in placenta tissue in GDM group were significant higher than those in the control group(P<0.05).③ There was positive correlation between serum visfatin level and HOMA-IR.Conclusion The increased visfatin in serum and placenta maybe involved in the development of GDM.更多还原