【摘要】 目的研究藤黄酸对人乳腺癌细胞MCF-7端粒酶活性和端粒酶逆转录酶(hTERT)mRNA表达及其转录调控基因C-myc的影响,以探讨藤黄酸对人乳腺癌细胞端粒酶活性的调控机制。方法藤黄酸作用于人乳腺癌细胞24h、48h、72h后,收集细胞,四甲基偶氮唑盐(MTT)比色法检测MCF-7细胞生长抑制率,同时采用PCR-ELISA法检测端粒酶活性、RT-PCR法检测hTERT mRNA的表达、Western blot技术检测C-myc蛋白的表达。结果藤黄酸能明显抑制人乳腺癌细胞MCF-7生长和增埴,对端粒酶活性、hTERT mRNA及转录调控基因C-myc的表达具有抑制作用,并呈明显的时效和量效相关性。结论藤黄酸能够明显抑制人乳腺癌细胞MCF-7增殖,其机制可能是对MCF-7细胞的端粒酶活性及其端粒酶转录调控基因C-myc有较好的抑制作用,提示藤黄酸有可能成为高效低毒的抗肿瘤天然药物。更多还原

【Abstract】 Objective To study the effect and potential mechanism of gambogic acid(GA) on telomerase and its key regulated gene C-myc in human breast cancer cells. Methods Telomerase activity was examined by PCR-ELISA and reverse transcriptase was detected by RT-PCR. Western blot assay was used to observe the C-myc protein expression. Results GA induced significant inhibition on MCF-7 cells(MTT).Telomerase activity and hTERT mRNA expression were both decreased greatly in a dose-dependent manner by Curcumin. There was a correlation between the expression of the hTERT mRNA and C-myc protein. Conclusions GA could significantly inhibite the activity of hTERT and the expression of oncogene C-myc in cultured MCF-7 cells, and this effect may induce the suppression on telomerase and hTERT mRNA in vitro.更多还原