【摘要】 目的:建立主导管结扎的SD大鼠下颌下腺组织损伤模型,观察主导管结扎及再通后腺体的组织学变化。方法:双重结扎SD大鼠下颌下腺主导管。部分腺体在6 d后摘取;其他腺体于6 d后松开结扎使再通,并于4、6、12、24 d后处死动物,取出腺体。通过HE及免疫组织化学染色观察其组织化学变化。结果:主导管结扎6 d后,腺体中腺泡消失,导管样结构增生,导管细胞表达CK19;在增生导管及周围见α6β1、LN阳性细胞。松开结扎再通的导管在6 d后有成熟的腺泡细胞出现,24 d后有大量成熟腺泡形成,Amylases、PAS(periodic acid-schiff)、AQP-5均在腺泡细胞中表达。结论:导管结扎损伤后腺体腺泡萎缩,导管上皮增生。松开结扎再通后腺泡细胞再生、功能恢复。更多还原

【Abstract】 Objective: To observe the histologic changes when the main duct of submandibular gland was ligated and released in SD rats.Methods: The main duct of submandibular gland was reopened after double ligation for 6 days.Some ligated submandibular glands were harvested at day 6 after ligation.Others were reopened and harvested 4,6,12 and 24 days after releasing respectively.The histological changes were observed by HE staining and immunohistological staining.Results: After the main duct ligation,acinar cells disappeared with ductal cell proliferation that expressed CK19.The α6β1,LN positive cells were found in proliferative duct and periductal areas.Acinar cells was appearing at day 6 after release of the ligation.24 days after releasing,large number of acinar cells expressing amylases,PAS and AQP-5 were formed.Conclusion: After duct ligation,acinar cells disappear and duct epithelium cells proliferate.After release of the ligation,the acinar cells regenerate and the function of the gland recovers.更多还原