【摘要】 采用Dikma C18(250mm×4.6mm,5μm),以10mmol/mL KH2PO4水溶液(pH 4.4)-甲醇为流动相梯度洗脱(流速∶1mL/min)在260nm波长、柱温25℃条件下用HPLC考察尿嘧啶、尿苷、腺嘌呤以及鸟苷在不同品种灵芝中的含量差异。结果表明,不同品种的灵芝中四种核苷类成分的含量存在显著差异,而且这种方法简便快速,稳定性和重复性良好,适用于灵芝中尿嘧啶、尿苷、腺嘌呤以及鸟苷含量的同时测定。更多还原

【Abstract】 An HPLC-based method for the accurate determination of uracil,uridine,adenine and guanosine in aqueous extracts of fruit bodies of different Ganoderma lucidum strains is described.Optimal separation of the four components was achieved by loading samples on to a Dikma C18(250mm×4.6mm,5μm)column and eluting with methanol-phosphate buffer(10mmol/mL,pH 4.4)using gradient elution at 25℃ with a flow rate of 1mL/min.Data revealed significant differences in analyte concentrations among the test G. lucidum strains.更多还原