【摘要】 目的 建立载脂蛋白E基因敲除(ApoE^-/-)小鼠颈动脉粥样硬化模型,并应用7.0T MRI对动脉粥样硬化斑块进行评价。方法 通过高脂饲养以及在左侧颈总动脉外置套管的方法建立实验组ApoE^-/-小鼠和对照组野生型C57小鼠颈动脉粥样硬化模型（n=6）,8周后对2组小鼠行MR扫描。扫描完成后取血测定血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白（HDL）和低密度脂蛋白（LDL）,并取损伤侧颈动脉行HE染色观察病理学变化,并对有斑块形成的血管行Oil Red O染色观察斑块内脂质成分的分布。结果 实验组小鼠在建模8周后MRI表现为损伤侧血管壁信号增高、管腔不规则狭窄,相应的组织病理学检查显示损伤侧动脉管腔狭窄,其内可见粥样斑块形成;而对照组小鼠在建模8周后MRI表现仅见2例血管壁信号增高、管腔狭窄,相应的组织病理学检查显示损伤侧动脉管壁增厚,未见粥样斑块形成。实验组小鼠血清TC和LDL值明显升高（t值分别为9.99和9.12,P值均<0.01）,TG和HDL值无明显变化（t值分别为-1.71和-1.05,P值>0.05）。结论 高脂饲养结合颈总动脉外置套管的方法可以快速建立ApoE^-/-小鼠动脉粥样硬化模型,应用7.0T MRI可成功对动脉粥样硬化斑块进行评价。更多还原

【Abstract】 Objective To establish the ApoE ’ carotid atherosclerosis mice model and to visualize the atherosclerotic plaque by7.0T MR scanner.Methods High fat diet were fed and silastic collars were placed around the left carotid artery of ApoE^-/- mice and wild type C57 mice to establish carotid atherosclerosis model（n=6）.The atherosclerotic plaque formation in the left carotid were observed by MRI after eight weeks,and blood samples were taken to measure serum total cholesterol（TC）,triglyceride（TG）,high-density lipoprotein（HDL） and low-density lipoprotein（LDL） and the left carotid artery specimens were taken to stain with HE to observe the pathological changes,and the plaques were taken to stain with Oil Red O to observe the fat.Results The vessel wall of the left carotid showed hyperintensity and the lumen were irregularly narrowed on MRI in experimental group after 8 weeks.And the histopathological examination showed atherosclerotic plaque in the vessel wall of the left carotid and the lumens were irregularly narrow.In the control group,only two cases showed hyperintensity in the vessel wall of the left carotid and irregularly narrow lumens by MRI,and the histopathological examination showed thickened arterial wall with no atherosclerotic plaque formation in the iumen of carotid.TC and LDL levels of experimental group were significantly higher than those of control group（t=9.99 and 9.12,respectively,P<0.01）,and the TG and HDL values showed no significant between-group difference（t=-1.71 and-1.05,P>0.05）.Conclusion Carotid artery atherosclerosis of ApoE^-/- mice can be quickly established through high fat diet and placing carotid collar,and 7.0T MRI can successfully evaluate the carotid atherosclerotic plaque.更多还原