Objective To construct the prokaryotic expression vector for 3C gene of enterovirus 71(EV71),express in E.coli and purify the expressed product.Methods The 3C gene of EV71 was amplified by PCR using the reverse transcription product of the virus as template,and cloned into vector pGEx-4T-1.The constructed recombinant plasmid pGEx-3C was transformed into E.coli BL21(DE3)for expression under induction of IPTG.The expressed recombinant fusion protein GST-3C was purified by GST-Agarose affinity chromatography a...
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