Objective The clone the gene encoding G protein of rabies virus(RV)4aG strain and compare its sequence with those of other vaccine strains so as to provide a theoretical basis for preparation of rabies vaccine in China.Methods G gene was amplified from RV 4aG strain and sequenced,based on which a phylogenetic tree was plotted by using bioinformatics software,and the functional site,secondary structure and B cell antigenic epitope of G protein were predicted and compared with those of other vaccine strains.R...
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