【摘要】 类芋螺毒素(CTX)基因存在于多种杆状病毒,编码富含半胱氨酸的多肽。从AcMNPV中克隆了ctx基因,利用AcMNPV杆粒操作系统构建了重组病毒Ac-pFastBac1-Pie1-ctx-eGFP和Ac-pFastBac1-Pie1-ctx。绿色荧光蛋白介导的亚细胞定位显示AcMNPV CTX定位于Tn细胞的细胞膜;抑菌实验表明,重组病毒Ac-pFastBac1-Pie1-ctx感染的胞外产物对金黄色葡萄球菌、微球菌、短小芽孢杆菌等临床致病菌具有显著的抗菌活性,但其作用机制尚需进一步阐明。更多还原

【Abstract】 Conotoxin-like(CTX) gene encodes a small cysteine-rich peptide in various baculoviruses.In this study,the ctx gene was cloned from AcMNPV,and the recombinant AcMNPVs(Ac-pFastBac1-Pie1-ctx-eGFP and Ac-pFastBac1-Pie1-ctx) was generated using AcMNPV bacmid system.Green fluorescence in CTX-eGFP-positive Tn cells was mainly observed on the cell membrane.The results also indicated that extracullular products of Tn cells infected by Ac-pFastBac1-Pie1-ctx had antimicrobial activity to some clinical pathogenic bactera(Staphylococcus aureus,Micrococcus and Bacillus pumilus).更多还原